蚯蚓蛋白对NIH3T3细胞的促进增殖作用研究  

The proliferation effects of Eisemia Foetida protein on NIH3T3 cells

作　　者：周莹[1] 宋淑亮[2] 梁浩[2] 王允山[2] 王伟莉[2] 吉爱国[1,2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东大学威海国际生物技术研发中心,山东威海264209

出　　处：《中国生化药物杂志》2010年第3期192-194,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨蚯蚓蛋白(EFP)对NIH3T3细胞的促进增殖作用。方法 MTT法测定EFP对大鼠成纤维细胞(NIH3T3)的促进增殖作用;细胞划痕法测定EFP促进NIH3T3细胞划痕创面愈合作用;测定NIH3T3细胞培养液中胶原降解产物羟脯氨酸的含量。结果 EFP具有促进NIH3T3细胞的增殖作用,促进划痕创面愈合作用,增加了NIH3T3细胞培养液中羟脯氨酸的含量。结论 EFP具有促进NIH3T3细胞增殖和划痕创面愈合作用。Purpose To investigate the proliferation effect of Eisemia Foetida protein （ EFP ） on NIH3T3 cells. Methods The cell proliferation vitality of EFP on NIH3T3 was assayed by MTT method. The function of EFP to promote injured area cicatrization was assayed by cell scratch experiment. The contents of the hydroxyproline （HYP） in the cell nutrient fluid was analized. Results EFP has the proliferation effect and the promoting injured area cicatrization effect on NIH3T3 cells, and also increases the HYP contents in the cell nutrient fluid. Conclusion EFP has the proliferation effect and the promoting in- jured area cicatrization on NIH3T3 cells.

关 键 词：蚯蚓蛋白 NIH3T3 羟脯氨酸 

分 类 号：R285[医药卫生—中药学]