HPLC法检测蛇莓中齐墩果酸的含量  

Determination of oleanolic acid in Duchesnea Indicae by HPLC method

作　　者：袁晓环[1] 王伟光[1] 李春彦[2] 武艳[1] 张羽飞[1] 初彦辉 

机构地区：[1]牡丹江医学院黑龙江省抗纤维化生物治疗重点实验室,黑龙江牡丹157011 [2]牡丹江医学院药学系,黑龙江牡丹157011

出　　处：《中国生化药物杂志》2010年第3期195-197,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：黑龙江省卫生厅项目(2009-006)

摘　　要：目的探讨HPLC测定蛇莓中齐墩果酸含量的方法。方法采用Thermo ODS-2HYPERTIL C18(250 mm×4.6 mm,5μm)色谱柱;流动相:0.065 moL/L NaH2PO4溶液-甲醇(14∶86);检测波长:210 nm;流速:0.8 mL/min。结果齐墩果酸在0.19～2.16μg范围内呈良好线性关系(r=0.998 2);平均回收率为98.6%,RSD(n=9)为1.68%。结论此法简单、准确、可靠,可以作为评价蛇莓质量的有效方法。Purpose To investigate the method about the determination of oleanolic acid in Duchesnea Indicae. Methods The chromatographic separation was performed on a Thermo ODS-2HYPER-TIL C18 （250 mm x4.6 mm,5 μm）. The column temperature was 25℃. The mobile phase consists of NaHzPO4 （0. 065 mol/L）-Methyl alcohol （14： 86）. Examination wave length was 210 nm. The flow rate was 0.8 mL/min. Results The liner relationship of oleanolic acid was good within the range of 0.19-2.16μg（r = 0. 998 2）. The average recovery was 98.6% and RSD（n = 9） was 1.68%. Conclusion The method is simple, accurate and reliable, and it can be used as an effective way to evaluate the quality of Duchesnea.

关 键 词：蛇莓 齐墩果酸 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]