三七对脐静脉内皮细胞增殖的影响  

Effect of Proliferation of HUVEC with Sanqi

作　　者：翟海英[1] 唐东昕[1] 田伟[2] 

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550002 [2]中国康复研究中心,北京100068

出　　处：《中国民族民间医药》2010年第11期47-47,49,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:观察三七以不同浓度和作用时间促脐静脉内皮细胞(HUVEC)增殖的作用。方法:将三七提取物分别按0.25mg/ml、0.5mg/ml、1mg/ml、2mg/ml、4mg/ml不同的浓度分为5个给药组和1个空白组。细胞培养后加入加入不同浓度的三七提取物100μl,分别作用24h、48h后,测定吸光A值。结果:24h时,2mg/ml作用最强,细胞增殖率=12.23%;48h时,4mg/ml作用最强,细胞增殖率(%)=27.93%。结论:三七提取物浓度为4mg/ml,作用时间48h,促HUVEC增殖作用最明显。Objective：To explore about Sanqi promoting the proliferation of HUVEC.Method：Prepared the Sanqi into 5 different groups： 0.25mg/ml,0.5mg/ml,1mg/ml,2mg/ml,and 4mg/ml,and with a control group.After 24h and 48h,test the colorimetric assay.Results：After 24h,2mg/ml showed the most cell proliferation,and the rate is as high as 12.23%%.And 48h later,4 mg/ml showed the strongest proliferation,the rate of 27.93%.Conclusion：The the most obvious proliferation showed while the concentration is 4 mg / ml,after the 48 h promoting HUVEC.

关 键 词：三七 HUVEC 细胞增殖 

分 类 号：R282.5[医药卫生—中药学]