高效液相色谱-串联质谱法测定苯巴比妥钠注射液中的有关物质  

Identification of Related Substances in Phenobarbital Sodium Injection by Esi-MS/MS

作　　者：孙煌[1] 付璀莹[1] 娄志红[1] 多凯[1] 姜连阁[1] 白政忠[1] 

机构地区：[1]黑龙江省药品检验所,哈尔滨150001

出　　处：《黑龙江医药》2010年第3期320-321,共2页Heilongjiang Medicine journal

摘　　要：目的:报道了苯巴比妥钠注射液中有关物质的高效液相色谱-串联质谱的鉴定方法。方法:采用高效液相色谱-串联质谱法测定苯巴比妥钠注射液中的有关物质。色谱柱为C_(18)柱,流动相为0.5%乙酸溶液-甲醇(40:60),流速为0.25ml/min;配有电喷雾离子源的三级四极杆型质谱仪。结果:根据色谱图、相对分子质量及二级质谱图确定1个降解产物。结论:本法能够排除辅料的干扰,确定其降解产物,为考察苯巴比妥钠注射液的稳定性和控制产品质量确定物质基础。Objective： A method was described for the identification of related substances in Phenobarbital Sodium Injection with LC-MS. Methods： HPLC-MS method was used to determine the relative substances in Phenobarbital Sodium Injection. The mobile phase was methanol-0.5% acetic acid （60：40）, flow rate was 0.25 mL/min; The method is more specific and accurate. It provides a scientific basis for the detection of stability of Phenobarbital Sodium Injection and the product quality under control.

关 键 词：高效液相色谱-串联质谱(LC-MS/MS)苯巴比妥钠注射液 有关物质 

分 类 号：R927.2[医药卫生—药学]