豆类丝核菌次级代谢产物对免疫抑制小鼠免疫功能的影响  

Effects of the secondary metabolites of rhizotonia leguminicola on immune functions of immunosuppressed mice

作　　者：朱超[1] 杨鸣琦[1] 赵守中[1] 

机构地区：[1]西北农林科技大学动物医学院,杨陵712100

出　　处：《中国免疫学杂志》2010年第5期392-395,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(30270979)

摘　　要：目的:研究豆类丝核菌次级代谢产物对环磷酰胺所致免疫抑制小鼠免疫功能的影响。方法:采用腹腔注射环磷酰胺(80mg/kg)制备免疫抑制模型,并给模型小鼠灌胃分别含苦马豆素(Swainsonine,SW)8、16、32mg/(kg.d)的豆类丝核菌次级代谢产物稀释液11天,同时设正常对照组和免疫抑制模型组,末次灌胃后24小时,眼眶采血,收集胸腺和脾,进行免疫学检测。结果:腹腔注射CTX80mg/kg,连续3天,可致小鼠免疫抑制,剂量为8、16mg/(kg.d)的SW组均可提高小鼠的外周血白细胞数,颉颃环磷酰胺所致的小鼠腹腔巨噬细胞吞噬功能的降低,提高外周血中溶血素水平、脾脏淋巴细胞转化以及NK细胞杀伤活性,尤其以SW16mg/(kg.d)最为明显。结论:豆类丝核菌次级代谢产物对环磷酰胺致免疫抑制小鼠的免疫功能有一定的恢复和增强作用。Objective：To study the effects of the secondary metabolites of Rhizotonia leguminicola on cyclophosphamide-induced immunoregulation in immunosuppressed mice.Methods：The immunosuppressed mouse model was established by cyclophosphamide（80 mg/kg） injected into abdominal cavity.The diluent of secondary metabolites of Rhizotonia leguminicola（SW） at the dosage of 8,16 and 32 mg/（kg·d） was intragastrically administered for 11 days respectively.Meanwhile,the normal control group and CTX model control group were established.Blood,thymus and spleen of the mice were collected respectively at 24 h after the last intragastric administration,and the immunological detection was also carried out.Results：CTX was injected constantly into the abdominal cavity at 80 mg/kg for 3 d,which leaded to immunosuppression of the mice.The groups which took SW at 8 and 16 mg/（kg·d） could increase the number of WBC of the mice.Cyclophosphamide induced a decrease of phagocytic activity of the peritoneal macrophages in the mice and a boost of hemolysin level of the peripheral blood,lymphocyte transformation of the spleens and the activity of NK cells,which were especially obvious in the group of SW at 16 mg/（kg·d）.Conclusion：The secondary metabolites of Rhizotonia leguminicola have effects of recovery and reinforcement in cyclophosphamide-induced immunoregulation of immunosuppressed mice.

关 键 词：豆类丝核菌 苦马豆素 环磷酰胺 免疫抑制 

分 类 号：S859.8[农业科学—临床兽医学]