毒热平注射液对流感病毒感染的巨噬细胞分泌NO的影响  

Effect of Dureping Injection on NO secreted by macrophage infected by influenza virus

作　　者：张艳丽[1] 王宁萍[1] 顾立刚[2] 李澎涛[2] 

机构地区：[1]宁夏医科大学病原生物学与免疫学系,银川750004 [2]北京中医药大学中医药抗病毒教育部重点实验室,北京100029

出　　处：《中国免疫学杂志》2010年第5期445-448,共4页Chinese Journal of Immunology

基　　金：宁夏医科大学特殊人才启动项目(XT200802)

摘　　要：目的:研究毒热平注射液对流感病毒感染的小鼠腹腔巨噬细胞株Ana-1细胞分泌NO的影响。方法:用100TCID50流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠腹腔巨噬细胞株Ana-1后换用6个不同浓度的含药维持液继续培养,分别于6、12、24和36小时收集细胞上清液,用Griess试剂法检测其释放NO的水平。同时提取1μg/ml药物组作用24小时后的细胞mRNA和核蛋白,利用RealTimePCR法和Westernblot技术,检测毒热平注射液对病毒感染前后巨噬细胞NF-κBp65mRNA及其蛋白表达水平的影响。结果:在药物作用的不同时间,60、30和10μg/ml各剂量组均能显著下调病毒感染巨噬细胞NO的分泌水平,且具有剂量依赖性;该药可明显下调病毒感染巨噬细胞NF-κBp65mRNA及其蛋白表达水平。结论:毒热平可通过下调细胞核因子-κB活性,抑制病毒感染巨噬细胞NO的分泌,发挥抗病毒感染作用。Objective：To survey the effect of the Dureping Injection on the kinetic change of nitrous oxide（NO） in macrophage infected by the influenza virus FM1 strain.Methods：The murinal celiac macrophage（Ana-1） was infected by the virus,add the different concentration of the drug in the supernatant of the macrophage for 6 h,12 h,24 h and 36 h.The level of the NO in the supernatant was measured by the method of traditional Griess.Ana-1 cell line was infected by influenza virus FM1 strain,then treated with Dureping Injection 1 μg/ml for 24 h.The cells were then collected,mRNA was extracted and the expression of NF-κB p65 was measured by RT-PCR;The nuclear protein was extracted and the expression of NF-κB p65 measured by Western blot.Results：60 μg/ml,30 μg/ml,10 μg/ml and 1 μg/ml group of Dureping Injection down-regulated amount of NO secreted by the Ana-1 cells infected by virus,and it was showed in dose relationship significantly;1 μg/ml group of Dureping Injection down-regulated the expression of the NF-κB p65 mRNA and protein.Conclusion：Dureping Injection has an obvious effect against influenza virus FM1 strain by depressing the activity of NF-κB,which prevents the production of NO secreted by Ana-1 cells infected by the virus.

关 键 词：毒热平 流感病毒 巨噬细胞 NF-ΚB NO 

分 类 号：R392.5[医药卫生—免疫学] R285.5[医药卫生—基础医学]