高效液相色谱法测定蒲公英提取物中咖啡酸含量  

Determination of Coffeic acid in Extract of Herba Taraxaci by High Performance Liquid Chromatographic Method

作　　者：贝京[1] 于光福 

机构地区：[1]江苏省生产力促进中心,江苏南京210042 [2]南京逐陆医药科技有限公司,江苏南京210061

出　　处：《中国野生植物资源》2010年第3期54-56,共3页Chinese Wild Plant Resources

摘　　要：建立了一种高效液相色谱法测定蒲公英提取物中咖啡酸的含量。色谱柱为DiamonsilTM(钻石)C18(4.6mm×150mm,5μm),流动相为甲醇-磷酸盐缓冲液(1.56g磷酸二氢钠/1000mL水,磷酸调节pH值3.8～4.0)(体积比为23∶77);检测波长为323nm,柱温为40℃。研究结果表明:线性范围为2.44～14.64mg/L,回归方程为y=41742x+2287.9相关系数为R2=0.9996,加标平均回收率为97.5%。本方法具有灵敏度高、重现性好、准确度高、操作简便等特点。A high performance liquid chromatographic eethod has been developed for determination of coffeic acid in extract of herba taraxaci. The chromatographic conditions were as follows：Diamonsil^TM C18（4.6mm×150mm,5μm） with methol H3PO4（ 1.56 g NaH2PO4/1000 mL water, pH3.8 -4.0） buffer（23：77, volume） as mobile phase and detector at 323 nm. The calibration curve showed good linearity （R^2 =0.999 6）. And the averae recovery of the method was 97.5%. Sensitive, reproducible and accurate, this method is particularly suitable for detemaination of coffeic acid in extract of herba taraxaci.

关 键 词：高效液相色谱法 蒲公英提取物 咖啡酸 

分 类 号：R284.1[医药卫生—中药学]