差异凝胶电泳技术的发展及其在生物学领域的应用  

Progress of Two-dimensional Difference Gel Electrophoresis and Its Application in Biology

作　　者：高宇[1,2] 陈利珍[2] 梁革梅[2] 迟德富[1] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《生物技术通报》2010年第6期65-70,共6页Biotechnology Bulletin

基　　金：转基因重大专项(2008ZX08011-002);国家"973"课题(2007CB109204);国家自然科学基金(30971921)

摘　　要：差异凝胶电泳技术是建立在双向电泳技术基础之上、采用3种荧光染料同时对蛋白样品进行标记、进而进行蛋白质表达量差异统计学分析的技术,具有更高的敏感度以及精确的定量能力。综述了差异凝胶电泳技术的基本原理、优缺点,概括了该技术在国内外生物学领域的应用现状,并对该技术的未来发展前景进行了展望。Two dimensional difference gel electrophoresis（2D-DIGE）is a developed technology based on 2-DE,which could statistical analyse the differences of protein expression by labeling the protein samples with three fluorescent CyDyes at same time.This technology has higher sensitivity and more accurate quantitative ability.The article reviews the basic principle,the advantages and disadvantages of 2D-DIGE,summarizes the present major applications in biological field at home and abroad,and prospects its developing foreground in the future.

关 键 词：蛋白质组学 差异凝胶电泳 荧光染料 

分 类 号：Q503[生物学—生物化学]