使用爪蟾卵母细胞载体研究黄连素对Kv1.4通道C型失活的影响  

Effects of berberine on C-type inactivation of Kv1.4 channel using Xenopus oocytes as carriers

作　　者：金鑫 舒周伍[2] 李晓艳[3] 黄东轩 

机构地区：[1]广东省深圳市宝安区观澜人民医院,广东深圳518110 [2]汕头大学医学院第一附属医院,广东汕头515041 [3]武汉大学人民医院,湖北武汉430060

出　　处：《现代中西医结合杂志》2010年第19期2358-2361,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的以非洲爪蟾卵母细胞为载体研究黄连素对去N端Kv1．4（Kv1．4AN）通道C型失活特性的影响。方法将Kv1．4AN的mRNA注射入非洲爪蟾卵母细胞并于18—20℃下孵育，成功表达后使用双微电极钳制法记录电流，观察黄连素对Kv1．4AN电流失活、复活的影响。结果黄连素对Kv1．4AN峰电流的抑制作用呈浓度依赖性和电压依赖性。黄连素可显著加速Kv1．4通道的C型失活速度，通道失活后的恢复时间延长。结论黄连素能显著加快Kv1．4通道的C型失活速度并延长其恢复时间，其机制可能为黄连素改变了Kv1．4通道S，或S6区域的构象：Objective It is to investigate the effects of berberine on C -type inactivation of Kvl. 4 channel with N point removed （Kvl. 4AN） using Africa Xenopns oocytes as carriers. Methods mRNA of Kvl. 4AN was injected into Xenopus oocytes and they were hatched under 18 -20 ~C. The current was recorded with two-electrode voltage-clamp technique（TEV） after successful expression to observe the effect of berberine on the inactivation and reactivation of Kvl. 4AN. Results The inhi- bition of berberine on Ipeak of Kvl. 4AN was voltage-dependent and concentrate-dependent. Berberine could markedly en- hanced the C -type inactivation of Kvl. 4 channel, and it could also slow the rate of channel recovery. Conclusion Berberine may enhance the C - type inactivation of Kvl. 4 channel and prolong recovery time. The mechanism is likely that berberine may change the crystal structure of S5 or S6. of the channels.

关 键 词：黄连素 Kv1.4 C型失活 爪蟾卵母细胞 

分 类 号：R965[医药卫生—药理学]