右归饮对大鼠激素性股骨头坏死局部脂联素表达的影响  

The Influence of You Gui Yin on The Local Adiponectin Expression of Steroid-induced Osteonecrosis of Femoral Head of Rats

作　　者：江彬锋[1] 方剑利[1] 俞楠泽[1] 肖鲁伟[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中医正骨》2010年第5期11-14,共4页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：国家自然基金资助课题(编号:30672702)

摘　　要：目的:研究补肾助阳中药右归饮对大鼠激素性股骨头坏死模型脂联素的影响。方法:取Wistar大鼠30只,随机分为空白对照组、模型组、右归饮治疗组,采用内毒素和甲基强地松龙肌肉注射诱导造成激素性股骨头坏死模型后,右归饮治疗组给右归饮灌胃,空白对照组和模型组以等量生理盐水灌胃治疗,于6周后手术取股骨头,观察股骨头病理形态,计算空骨陷窝率,利用基因芯片技术检测股骨头基因表达,并利用RT-PCR技术检测脂联素基因adipoq的表达情况。结果:①HE染色显示右归饮治疗组较模型组骨膜变完整,同源软骨细胞数增加,骨小梁变整齐,软骨下血管变丰富。②模型组空骨陷窝率最高,右归饮治疗组次之,空白对照组最低。空白对照组、右归饮治疗组的空骨陷窝率与模型组相比较,差异均有统计学意义(P<0.01)。③基因芯片表达结果显示,空白对照组与模型组相比较,脂联素基因adipoq无明显差异(Ratio值<2.0);右归饮治疗组与模型组相比较,脂联素基因adipoq明显上调(Ratio值>2.0)。结论:在大鼠激素性股骨头坏死模型模型中,糖皮质激素联合内毒素造模对股骨头局部的脂联素表达无明显影响;补肾助阳中药右归饮能明显刺激股骨头局部的脂联素的表达。Objective：To explore the mechanism of You Gui Yiu, a kidney-supplementing and yang-activating formula, in preventing and treating steroid-induced femoral head necrosis （SIFHN）of rats. Methods ：30 Wistar rats were involved, and were randomly divided into the blank control group （ group A） , the model group （ group B） and the You Gui Yin group （ group C）. SIFHN models were established by injection of colibacillus endotoxin and prednisolone intramuscularly. Group C was lavaged with You Gui Yin, while group A and group B were lavaged with the same amount of saline. All the animals were sacrificed at the end of the 6th week for obtaining femoral heads. HE stai- ning was used for observing morphological changes and calculating the rate of empty lacunae. Gene chip technology was used for detecting gene expressions. RT-PCR was used for detecting the expression of adiponectin gene, adipoq. Results：①HE staining showed that, compared to group B, group C possessed better periosteum integrity,increased number of homologous cartilage cells,more orderly bone trabeeulae and richer subchondral blood vessels. ②Group B had the most empty lacunae, group C lesser and group A the least. The difference between group B and group A or group C was of statistical significance （ P 〈 0.05 ）. ③Gene chip showed that, compared to group B, group C had up- regulated gene expression of adiponectin gene,adipoq （Ratio 〉 2.0） ;compared to group B,group A had no significant difference in the ex- pression of adipoq （ Ratio 〈 2.0 ）. Conclusion ： In the steroid-induced osteonecrosis of femoral head models of rats, colibaeillus endotnxin and prednisolone had no significant influence in adiponectin expression. You Gui Yin has a stimulating function of adiponectin gene expres- sion of femoral heads.

关 键 词：股骨头坏死 脂联素 右归饮 基因 大鼠 

分 类 号：R285.5[医药卫生—中药学]