RP-HPLC测定红毛五加中的异嗪皮啶  

Determination of isofraxidin from Acanthopanacis giraldii by RP-HPLC

作　　者：李莹[1] 刘圆[1] 袁玮[1] 刘永恒[1] 任烨[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041

出　　处：《华西药学杂志》2010年第3期339-341,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省中医药管理局科技专项(2008-32);国家科技支撑计划重点项目(2007BAI25B05);四川省青年基金项目(07ZQ026-011)

摘　　要：目的建立测定红毛五加药材中异嗪皮啶的方法并比较不同药用部位及不同产地、不同采收期的红毛五加皮中异嗪皮啶的含量。方法采用HPLC法,流动相为乙腈-甲醇-0.1%磷酸水溶液(20:10:70),流速为1mL·min-1,检测波长为343nm,柱温为25℃。结果异嗪皮啶1.59×10-3～0.80μg与峰面积具有良好的线性关系,回归方程为:Y=2.636×104X-1.051×103(r=0.9997,n=8),平均回收率为99.15%,RSD=0.95%(n=5)。结论所用方法简便、准确、重复性好,可控制红毛五加药材的质量。OBJECTIVE To establish an RP-HPLC method to determine isofraxidin in Acanthopanax giraldii Harms and to investigate contents of isofraxidin from different medical parts,different habitats and different collection time of Acanthopanax giraldii Harms.METHODS The analysis was carried out at 25 ℃ on a Kromasil C18 column.The mobile phase consisted of acetonitrile-methano1 0.1% phosphoric acid（20：70：10） and flow rate of 1.0 mL·min-1.The detective wavelength was 343 nm.RESULTS The calibration curve was linear within the concentration ranges of 1.59 × 10-3 to 0.80 μg.The average recovery was 99.15% with RSD of 0.95%.CONCLUSION This method is simple,accurate and replicate.And it can be adopted for the quality control of Acanthopanax giraldii Harms.

关 键 词：红毛五加 异嗪皮啶 反相-高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]