HPLC测定楤木花中的3,5-二咖啡酰奎宁酸  

Determination of 3,5-dicaffeoylquinic acid in Aralia chinensis flower by HPLC

作　　者：何兵[1] 田吉[1] 刘艳[1] 肖顺汉[1] 

机构地区：[1]泸州医学院药物研究所,四川泸州646000

出　　处：《华西药学杂志》2010年第3期365-366,共2页West China Journal of Pharmaceutical Sciences

基　　金：泸州市指导性项目(泸市科[2007]64号);四川省教育厅重点项目(No.2005A076)

摘　　要：目的建立测定楤木花中3,5-二咖啡酰奎宁酸含量的方法。方法采用HPLC法,色谱柱为Dikma Kromasil C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(23:77),检测波长为328nm。结果 3,5-二咖啡酰奎宁酸0.404～4.040μg与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为98.74%,RSD=0.62%(n=9)。结论所用方法简便、快速、准确,可作为测定楤木花中3,5-二咖啡酰奎宁酸含量的方法 。OBJECTIVE To establish an HPLC method for the determination of 3,5-dicaffeoylquinic acid in Aralia chinensis flower.METHODS The separation was carried out on a Dikma Kromasil C18 column（250 mm × 4.6 mm,5 μm）.The mobile phase was acetonitrile-0.1% phosphoric acid（23：77）.The detective wavelength was set at 328 nm.RESULTS The calibration curve showed a good linearity over the range of 0.404-4.040 μg（r = 0.9999）.The average recovery was 98.74% with RSD of 0.62%.CONCLUSION This method is simple,rapid,accurate and can be used as a quantitative analysis method for Aralia chinensis flower.

关 键 词：楤木花 3 5-二咖啡酰奎宁酸 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]