RP-HPLC测定金顶侧耳中的烟酸  

Determination of nicotinic acid in Pleurotm citrinopileatus by ionpair RP-HPLC

作　　者：刁继红[1] 刘佳[2] 张善玉[2] 

机构地区：[1]延边大学附属医院药剂科,吉林延吉133000 [2]长白山生物资源与功能分子教育部重点实验室,吉林延吉133000

出　　处：《华西药学杂志》2010年第3期371-372,共2页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金(批准号:30860343)

摘　　要：目的建立金顶侧耳中烟酸含量的测定方法。方法采用RP-HPLC法,色谱柱为C18柱,流动相为甲醇-0.004mol·L-1庚烷磺酸钠-乙酸(5:95:0.2),检测波长为263nm。结果烟酸对照品0.08～0.40μg与峰面积呈良好的线性关系(r=0.9999,n=5),样品的平均回收率为103.84%,RSD=2.80%(n=9)。结论所建方法简便、准确、重复性好,可测定金顶侧耳中烟酸的含量。OBJECTIVE To establish an HPLC method for the determination of nicotinic acid in Pleurotm citrinopileatus Sing..METHODS Ionpair reversed-phase（RP） high performance liquid chromatography（HPLC）was performed using a C18 column with methanol-0.004 mol·L-1 heptane mahogany acid sodium-acetic acid（5：95：0.2）as mobile phase.The detection wavelength was set at 263 nm.RESULTS The calibration curve of nicotinic acid was linear from 0.08 to 0.4 μg（r = 0.9999）.The average recovery was 103.84% with RSD of 2.80%.CONCLUSION This method was proved to be accurate and simple.And it can be selected to control the quality of Pleurotm citrinopileatus Sing.

关 键 词：金顶侧耳 离子对 烟酸 HPLC 含量测定 

分 类 号：R917[医药卫生—药物分析学]