复方胆通片和十滴水中土大黄苷的检测  

Detection of rhaponticin illegally mixed in Compound Dantong tablets and Shidishui tincture by TLC and HPLC

作　　者：申兰慧 杨敏智 梅雪艳 

机构地区：[1]江苏省无锡药品检验所,江苏无锡214021

出　　处：《华西药学杂志》2010年第3期377-378,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立TLC结合HPLC法检查复方胆通片、十滴水中的土大黄苷的方法。方法采用硅胶G板,展开剂为乙酸乙酯-丁酮-甲酸-水(10:7:1:1),在365nm下检视;采用Lichrospher C18柱(250mm×4.6mm,5μm),流动相为乙腈-甲醇-水(7:30:63),检测波长为320nm。结果复方胆通片、十滴水中非法掺入土大黄的指标性成分土大黄苷在365nm下显持久的蓝紫色荧光斑点,通过HPLC法得到进一步验证。结论所建方法简便、快速、专属性强,能有效地检查复方胆通片、十滴水中是否掺有土大黄。OBJECTIVE To establish a method to detect the rhaponticin illegally mixed in Compound Dantong tablets and Shidishui tincture by TLC and HPLC.METHODS Silica gel（G）TLC was used with a mixture of ethyl acetate-2-butanone-formic acid water（10：7：1：1）]as developer at detection wavelength of 365 nm.Lichrospher C18 column was used and the mobile phase consisted of methanol-acetonitrile-water（30：7：63）.Detective wavelength was 320 nm.RESULTS Rhaponticin displayed a constant blueviolet spot under 365 nm in Compound Dantong tablets and Shidishui tincture.It was tested and verified by HPLC.CONCLUSION The method is simple,rapid,accurate and suitable for detecting the rhaponticin illegally mixed in Compound Dantong tablets and Shidishui tincture.

关 键 词：复方胆通片 十滴水 土大黄苷 薄层色谱法 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]