转基因辣椒离体子叶再生研究  

Regeneration of Cotyledon Explants of Transgentic Pepper

作　　者：李长福[1] 张守鸿[1] 葛正龙[1] 范芳[1] 冯雪松 

机构地区：[1]遵义医学院生物化学与分子生物学教研室,贵州遵义563003 [2]贵州省湄潭县农业局,贵州湄潭564100

出　　处：《贵州农业科学》2010年第5期8-11,共4页Guizhou Agricultural Sciences

基　　金：遵义市重点项目科研基金"用基因工程技术培育辣椒新品种"[(2006)01-2]

摘　　要：探讨了不同浓度植物高活性制剂LCI、AA、GA3和NAA等对遵椒1号不定芽的诱导、伸长和生根各个阶段的影响。结果表明,最佳不定芽诱导分化培养基为：MS＋LC 1.0 mg/L＋IAA 0.1 mg/L＋ABA 0.3 mg/L＋Spm 100μmol/L＋AgNO35 mg/L＋PD 6 mL/L（pH 5.8-6.0）;最佳不定芽伸长培养基为：MS＋LC 0.1 mg/L＋IAA 0.2 mg/L＋GA31.0 mg/L＋Spm 100μmol/L＋AgNO35 mg/L＋PD 6 mL/L（pH 5.8~6.0）;最佳生根培养基为：1/2MS＋IAA 1.0 mg/L＋NAA 0.5 mg/L（pH 5.8-6.0）。The pepper cotyledons at different seedling age were cultured on MS with different LC,IAA,GA3 and NAA concentration and combination to study effects of different hormones concentration and combination on induction,elongation and rooting of adventitious buds cultured from pepper cotyledon explants.The results showed that the optimum medium for induction and differentiation of adventitious buds cultured from pepper cotyledon explants was MS＋LC 1.0 mg/L＋IAA 0.1 mg/L＋ABA 0.3 mg/L＋Spm 100 μmol/L＋AgNO3 5 mg/L＋PD 6 mL/L（pH 5.8～6.0）,the optimum medium for elongation of adventitious buds cultured from pepper cotyledon explants was MS＋LC 0.1 mg/L＋IAA 0.2 mg/L＋GA3 1.0 mg/L ＋Spm 100 μmol/L＋AgNO3 5 mg/L＋PD 6 mL/L（pH 5.8～6.0）,the optimum medium for rooting of adventitious buds cultured from pepper cotyledon explants was 1/2MS＋IAA 1.0 mg/L＋NAA 0.5 mg/L（pH 5.8～6.0）.

关 键 词：转基因 辣椒 子叶 植株再生 

分 类 号：S641.3[农业科学—蔬菜学]