低温预处理对烟草花药培养诱导率的影响  

Effect of Low Temperature Pre-treatment on Embryoid Induction Rate of Tobacco Anther Culture

作　　者：陈春艳[1] 刘仁祥[1] 雷红梅[1] 刘胜传[1] 王颖[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《贵州农业科学》2010年第5期16-18,共3页Guizhou Agricultural Sciences

基　　金：贵州省"十一五"重大专项"贵州烤烟种质资源创新及优质抗病新品种选育"[黔科合重大专项字(2006)6026];贵州省烟草专卖局(公司)"十一五"重大专项"烤烟新品种选育及配套技术研究"[黔烟科(2006-03)]

摘　　要：为了研究低温预处理不同时间对烟草花药培养胚状体诱导率的影响,以烟草K326×GDH黄叶突变体组合的F2、F3和F4植株的花药为试验材料,在接种前将花药用4℃低温预冷处理24h、48h和72h后,在(26±1)℃条件下进行花药培养。结果表明,烟草花药经4℃低温预处理48h后接种,可明显提高花药胚状体的诱导率,最佳培养世代为F2。The anthers of F2,F3 and F4 plants of K326×GDH（the mutant with yellow leaf） pretreated at 4℃ for 24h,48h and 72h were cultured at（26±1）℃ to study the effect of different low temperature time on embryoid induction rate of tobacco anthers.The results showed that culture of tobacco anthers pretreated at 4℃ for 48h could significantly increase the embryoid induction rate and the optimum culture generation was F2.

关 键 词：烟草 出苗率 低温处理 花药培养 

分 类 号：S572.01[农业科学—烟草工业]