应用促卵泡激素基因SINE标记测定大约克猪纯合度  

Application of SINE Marker in Homozygosis Determination of Yorkshire Pigs

作　　者：李升[1,2] 赵星艳[1] 张兆强[3] 王三铭[1] 王嘉福[1,3,2] 冉雪琴[3] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学农业生物工程省重点实验室,贵州贵阳550025 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2010年第5期140-142,共3页Guizhou Agricultural Sciences

基　　金：国家科技支撑计划项目"高寒山区高效循环养殖(优质猪)关键技术研究与集成示范"(2008BADB5B02);贵州省优秀青年科技人才培养计划项目"贵州地方猪种功能基因及遗传多样性研究"(2005-0506);贵州省科技创新人才团队建设专项项目"贵州省动物遗传育种与生物技术科技创新人才团队"(2009-4006);贵州省长资金专项项目"猪肌细胞生长分化相关基因及调节机制研究"(2009-100)

摘　　要：为了使猪种引进有便捷、准确的检测手段,采用聚合酶链式反应(Polymerase chain reaction,PCR)方法,检测了120头大约克种猪的纯合度。结果表明:受检母猪FSHβ基因的BB纯合基因型频率为82.5%,AB杂合基因型频率为17.5%,未检测到AA纯合基因型,经计算B等位基因的频率明显高于A等位基因频率。表明,该批次大约克猪存在一定比例的内二元或内三元杂交猪,应加强管理,严格控制再引种的纯度。该检测方法适用于区分国内地方猪品种和欧洲猪品种的纯合度,为引种种猪的纯合度检测提供准确的鉴定手段,有良好的应用前景。The homozygosis of 120 Yorkshire pigs was determined by polymerase chain reaction（PCR） to study the determination method with simplicity and precision for introduction of breeding pigs The results showed that the frequency of homozygous BB genotype and heterogenous AB genotype of tested farrowing pigs with FSH β gene was 82.5% and 17.5% respectively,the homozygous AA genotype was not determined,and the frequency of allele B was obviously higher than that of allele A,which indicated that there was some two-way or three-way pigs in the tested Yorkshire pigs.The PCR method can be used to identify the homozygosis of local pig varieties in China and introduced European pig varieties.Therefore,the PCR method has good application prospect in homozygous determination of introduced breeding pigs.

关 键 词：大约克猪 FSHΒ基因 分子标记 SINE 

分 类 号：S852.1[农业科学—基础兽医学]