苦苣菜总黄酮提取工艺及HPLC法测定芦丁的含量  

Extraction Technology of Total Flavonoids from Sonchus oleraceus and Determination of Rutin Content by HPLC

作　　者：覃成[1,2] 高健[2] 罗希榕[1] 

机构地区：[1]贵州省遵义市农业科学研究所,贵州遵义563102 [2]四川农业大学玉米研究所教育部作物基因资源与遗传改良重点试验室,四川雅安625014

出　　处：《贵州农业科学》2010年第5期199-201,共3页Guizhou Agricultural Sciences

摘　　要：采用正交试验方法考察乙醇体积分数、提取温度、提取时间、料液比对苦苣菜总黄酮提取效果的影响,寻求苦苣菜总黄酮提取最佳工艺,并进一步建立总黄酮中芦丁含量的高效液相色谱（HPLC）法测定方法。结果表明,料液比对总黄酮提取量有显著影响,总黄酮提取量也受到提取温度和乙醇浓度的影响。最佳提取工艺：30%乙醇作为溶剂,提取温度80℃,料液比1∶20,提取时间2 h,此条件下总黄酮提取量达6.065 mg/mL。HPLC法测定黄酮中芦丁含量结果表明,在0.025-0.075 mg/mL范围内线性关系良好（R=0.999,n=5）,平均回收率为97.98%,RSD为2.53%。HPLC法简便快捷、灵敏,结果准确,重现性好,可用于苦苣菜中芦丁含量的检测。The orthogonal design was used to study the effect of ethanol concentration,extract temperature,time and solid/liquid ratio on total flavonoids extract from Sonchus oleraceus.and establish a determination method of rutin content in total flavonoids by HPLC.The results showed that the solid/liquid ratio had the significant effect on total flavonoids quantity and the total flavonoids quantity was affected by extraction temperature and ethanol concentration also.6.065 mg/mL total flavonoids can be extracted under the optimum extract technological conditions of 30% ethanol,1∶20 solid/liquid ratio,at 80℃ for 2 h.The results of determining rutin content in total flavonoids by HPLC showed that there was a good linear relationship between peak area and rutin content with 0.025～0.075 mg/mL and its mean recovery and RSD were 97.98% and 2.53%,which indicates that HPLC with simplicity,sensitivity,accuracy and good repetition can be used in determination of rutin content in Sonchus oleraceus.

关 键 词：苦苣菜 总黄酮 芦丁 提取工艺 高效液相色谱法 

分 类 号：S567.9[农业科学—中草药栽培]