养殖用水中孔雀石绿和结晶紫及其代谢物的测定  

Determination of Malachite Green、Gentian Violet and Their metabolites in Aquatic Water

作　　者：马瑞欣 孙伟彬[3,2] 郎玉苗 石洁卿[3,2] 葛美丽 

机构地区：[1]河北省水产养殖病害防治监测总站,河北石家庄050011 [2]河北省水产品质量检验检测站,河北石家庄050801 [3]河北省水产技术推广站,河北石家庄050011

出　　处：《河北渔业》2010年第6期21-25,41,49,共7页

摘　　要：建立了用高效液相色谱紫外、荧光检测器同时检测养殖用水中的孔雀石绿、结晶紫及其代谢物隐色孔雀石绿和隐色结晶紫的分析方法。孔雀石绿和结晶紫用紫外检测器588nm波长检测；隐色孔雀石绿和隐色结晶紫用荧光检测器检测，激发波长：265nm，发射波长：360nm。孔雀石绿和结晶紫的线性范围5．0～2500μg／L；隐色孔雀石绿和隐色结晶紫的线性范围1．0～1000μg／L，四种物质的检出限均为0．5μg／L，回收率均在70％以上，RSD〈15％．此方法也可用于地下饮用水中孔雀石绿、结晶紫、隐色孔雀石绿和隐色结晶紫的检测。A high performance liquid chromatography （HPLC） method had been established for the simultaneous determination of malachite green （MG）, gentian violet （GV） and their metabolites： leucomalachite green（LMG）and leucogentian violet （LGV） in aquatic water. HPLC with ultraviolet detector （VWD）（λ=588 nm） was used for the determination of MG and GV while LMG and LGV were detected by fluorescence detector （λex=265 nm and Xerox360 nm）. The linear range of MG and GV was 5.0 μg/L-2 500μg/L and the good linearity of LMG and LGV was within 1.0 μg/L -1 000 μg/ L. The limits of detection were 0.5μg/L. The average recoverls were all above 70% and the relative standard deviations of recoveries were all below 15 %. The method also can be used for the determination of MG,GV,LMG and LGV in underground drinking water.

关 键 词：孔雀石绿 结晶紫 隐色孔雀石绿 隐色结晶紫 高效液相色谱法 养殖用水 

分 类 号：S948[农业科学—水产养殖]