杜鹃红山茶花粉保存及其生活力测定  

Pollen Preservation and Viability Test of Camellia azalea

作　　者：刘玉玲[1] 潘文[1] 张方秋[1] 徐斌[1] 朱报著[1] 王裕霞[1] 赵阳阳[1] 

机构地区：[1]广东省林业科学研究院,广州510520

出　　处：《广东林业科技》2010年第2期1-6,共6页Forestry Science and Technology of Guangdong Province

基　　金：十一五"国家科技支撑计划专题(2006BAD01A1802);广州市林业科技计划项目"杜鹃红山茶新品种选育

摘　　要：以5 a生盆栽杜鹃红山茶嫁接苗的花粉为试材,研究了不同培养基配方、不同培养时间对花粉发芽的作用,并探讨了不同保存条件和保存时间下花粉生活力的差异。结果表明:杜鹃红山茶花粉发芽率呈抛物线型,在2.5%琼脂+10%蔗糖的培养基上发芽率最高,8 h的培养时间为杜鹃红山茶花粉发芽的最适观测时间,4℃和-20℃保存都是适合杜鹃红山茶花粉长期保存的条件,但4℃保存更具有实践意义。Took the pollen of Camellia azalea,which were 5 d grafted seedlings,as experimental material,researched the effects of different culture media and different culture time on the role of pollen germination,discussed the differences of the pollen viability under different storage conditions and different storage time.The results showed that： the pollen germination rate of C.azalea showed parabolic,pollen on 2.5% agar ＋10% sucrose medium,the germination rate is the highest,after 8 h culture time is the the optimum observation time of pollen germination of C.azalea,stored at 4℃ and-20℃ were suitable conditions for long-term preservation of the pollen,however,stored at 4℃ was more practical significance for pollen preservation of C.azalea.

关 键 词：杜鹃红山茶 花粉 发芽率 保存 

分 类 号：S685.14[农业科学—观赏园艺]