肠系膜动脉缺血再灌注诱发多器官功能障碍综合征模型研究  

Research on Multiple Organ Dysfunction Syndrome Model Induced by Intestinal Ischemia-reperfusion in Rats

作　　者：朱枝祥[1] 陈丁丁[1] 

机构地区：[1]中国药科大学药理教研室,南京210009

出　　处：《抗感染药学》2010年第2期93-96,共4页Anti-infection Pharmacy

摘　　要：目的:研究肠缺血再灌注致多器官功能障碍综合征(MODS)模型,为筛选治疗MODS的药物建立稳定的动物模型和检测指标。方法:取20只SD大鼠,分成两组(每组10只):假手术组和模型组。假手术组:腹腔注射戊巴比妥钠(40mg/kg)麻醉后开腹,不做任何处理后关腹;模型组:腹腔注射戊巴比妥钠(40mg/kg)麻醉后开腹,夹闭肠系膜上动脉45min后再灌注后关腹制备模型,手术后观察大鼠一般状态,24h后检测两组动物肺指数、肺湿质量/干质量比、各脏器功能相关的血清生化指标、血清TNF-a、各组织器官中髓过氧化物酶(MPO)活力、血清和小肠组织二胺氧化酶(DAO)活力。结果:模型组:大鼠一般状态明显劣于假手术组;肺湿质量/干质量比,血清ALT、CRE、CK-MB、TNF-a指标,各组织器官中MPO活性,血清DAO活力均显著高于假手术组,而小肠组织匀浆上清液中DAO活力下降。结论:夹闭大鼠肠系膜上动脉45min后再灌注可成功制备MODS模型,血清ALT、CRE、CK-MB、DAO等组织器官功能损伤程度标志物和各组织器官匀浆上清液中MPO、血清TNF-a等全身性炎症反应标志物可以作为MODS的检测指标。Objective：To study MODS model induced by intestinal ischemia-reperfusion in rats,a stable animal model and detecting indexes were built.Methods：Randomly divided twenty SD rats into two groups（ten in every group）：sham and model.Anesthetized rats in sham group were treated with sodium pentobarbital （40 mg/kg）,cut open their abdominal cavity and closed without any treatment.Anesthetized rats in model group as well,superior mesenteric artery was closed for 45 minutes and reperfusion was made,then abdominal cavity closed.After operating,general conditions of rats were observed;24 hours later,the lung index,lung wet weight/dry weight,ALT,CRE,CK-MB,TNF-a in serum,MPO activities in supernatant of several key organs＇ homogenate,DAO activities in serum and supernatant of small intestine＇s homogenate were detected.Results：After operating,compared to sham group,rats in model group got worse under general conditions and lung wet weight/dry weight,ALT,CRE,CK-MB,TNF-a in serum,MPO activities in supernatant of several key organs＇ homogenate,DAO activities in serum rose significantly and DAO activities in supernatant of intestine＇s homogenate descended remarkably.Conclusion：Closing superior mesenteric artery for 45 minutes and made reperfusion could make MODS model successful.Some markers in serum representing the degree of injury of some key organs＇ function,such as ALT,CRE,CK-MB,DAO activities,and markers representing systemic inflammatory response,MPO activities in supernatant of several key organs＇ homogenate and TNF-a in serum,could be used as monitoring indexes of MODS.

关 键 词：肠缺血再灌注 多器官功能衰竭综合征(MODS) 动物模型 检测指标 

分 类 号：R965[医药卫生—药理学]