龙眼胚性愈伤组织2个乙烯受体基因的克隆及序列分析  

Cloning and Sequence Analyzing of Two Ethylene Receptor cDNAs from Dimocarpus longan Embryogenic Callus

作　　者：李惠华[1,2] 赖钟雄[1] 苏明华[2] 林玉玲[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]福建省亚热带植物研究所,福建厦门361000

出　　处：《热带作物学报》2010年第4期585-591,共7页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划(No.2007BAD07B01);国家自然科学基金(No.30471204);福建省重大科技平台建设(No.2008N2001)资助项目的部分内容

摘　　要：以龙眼胚性愈伤组织为材料,采用同源克隆和RACE方法分离得到两个乙烯受体基因的cDNA序列,即DL-ETR1和DL-ERS1,并在GenBank登录(检索号分别为FJ513322和FJ513323)。DL-ETR1全长为2611bp,包含长2223bp的完整的开放阅读框(ORF)以及388bp的3-UTR,3'poly(A)尾长24bp;DL-ERS1全长为2259bp,包含一个1929bp的ORF及330bp的3-UTR,3'poly(A)尾长17bp。两者根据核苷酸序列推测的蛋白质具有61.62%的同源性,在N端的同源性较C端高,DL-ERS1比DL-ETR1少104个氨基酸,DL-ERS1的蛋白在C端缺乏信号接受域,HisKA、ATPase_c以及GAF是它们共有的结构域,同属ETR1亚类乙烯受体。Two ethylene receptor cDNAs, DL-ETR1 and DL-ERS1, which were obtained from Dimocarpus longan Embryogenic Callus, were cloned with homologous cloning and RACE （Rapid amplification of eDNA ends）. The two fragments had been submitted to the DDBJ/EMBL/GenBank databases, the accession numbers were FJ513322 and FJ513323, respectively. The full length of DL-ETR1 eDNA , about 2611 bp [including a 24 bp poly （A） tail] consisted of an open reading frame of 2 223 bp , and 3＇untranslated regions of 388 bp, while the full length of DL-ETS1 eDNA , about 2 259 bp [including a 17 bp poly（A） tail] consisted of an open reading frame of 1 929 bp , and 3＇untranslated regions of 330 bp. Both of the putative proteins, which had three conserved regions： HisKA, ATPase_e and GAF domains, belonged to the ETR1 sub-groups. And, their homology were 61.62%, their homology of N-terminal amino acids was higher than that of Cterminal end. Moreover, DL-ERS1 lacked 104 amino acids and a signal receptor domain at C-terminal end than DL-ETR1.

关 键 词：龙眼胚性愈伤组织 乙烯受体 基因克隆 序列分析 

分 类 号：S667.2[农业科学—果树学]