蝴蝶兰丛生芽途径的组织培养技术  

Micropropagation of Phalaenopsis with Clustered Shoots in Vitro

作　　者：李金雨[1] 洪丽萍[1] 

机构地区：[1]福建省亚热带植物研究所,厦门361006

出　　处：《热带作物学报》2010年第4期610-613,共4页Chinese Journal of Tropical Crops

基　　金：福建省科技攻关计划资助项目(No.2002N022)资助

摘　　要：对蝴蝶兰丛生芽途径的组织培养进行研究,结果表明,不经过愈伤组织直接诱导丛生芽的过程中,6-BA是影响蝴蝶兰丛生芽诱导的重要因素,6-BA浓度达3.0mg/L以上时丛生芽的诱导率就可达100%;在丛生芽增殖过程中,6-BA8.0mg/L处理效果最好,丛生芽增殖率可达304%;6-BA8.0mg/L+NAA0.5mg/L的处理更能促进蝴蝶兰丛生芽的增殖效果,丛生芽增殖率可达506%;生根培养基组合MS+NAA0.5mg/L+10%椰子汁的生根效果较好。The tissue culture of Phalaenopsis clustered shoots in the study showed that O-BA was an important factor in inducing Phalaenopsis clustered shoots, which could lead to an induction rate of clustered shoots of 100% when the concentration of 6-BA reached 3.0 mg/L or more. During the proliferating period of clustered shoots, the treatment of 6-BA 8.0 mg/L showed the best effect with a proliferating rate of clustered shoots of 304%. The treatment of 6-BA 8.0 mg/L ＋ NAA 0.5 mg/L promoted the effect of proliferating of clustered shoots with a proliferating rate of clustered shoots of 506%. In rooting stage, the MS medium with NAA 0.5 mg/L ＋10% coconut juice was efficient.

关 键 词：蝴蝶兰 丛生芽 组织培养 

分 类 号：S682.31[农业科学—观赏园艺]