檀香萜烯合成酶基因的克隆与序列分析  

Cloning and sequence analyzing of terpene synthase gene in Santalum album L.

作　　者：文海涛[1] 赵红英[1] 林励[1] 邱贤秀[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广东药学院学报》2010年第2期131-133,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：高等学校博士学科点专项科研基金(20094425110009)

摘　　要：目的通过克隆技术获得参与檀香挥发油形成的萜烯合成酶基因。方法利用CTAB-LiCl法提取檀香已结香心材总RNA,采用RT-PCR技术克隆萜烯合成酶基因。结果克隆获得了一个檀香萜烯合成酶基因,该基因编码区全长1 731 bp,编码576个氨基酸残基。结论 CTAB-LiCl法能提取较高质量的檀香心材总RNA,克隆获得的萜烯合成酶具有编码区。Objective To clone the terpene synthase gene from Santalum album L.which participates in the synthesis of essential oil.Methods CTAB-LiCl treatment was used to extract total RNA from the heartwood of Santalum album L.And RT-PCR was employed to clone the terpene synthase gene at the same time.Results One terpene synthase gene consisted of 1 731 bp nucleic acid was cloned from Santalum album L.,which encodes 576 amino acid residues.Conclusion CTAB-LiCl treatment could extract high quality RNA from the heartwood of Santalum album L.,and the cloned terpene synthase gene had the open reading frame.

关 键 词：檀香 萜烯合成酶 基因克隆 序列分析 

分 类 号：S567.19[农业科学—中草药栽培]