RP—HPLC法测定(7-甲氧基-3,4-二氢-1-萘基)乙腈的含量及其有关物质  

RP-HPLC determination of content of (7-methoxy-3,4-dihydro-1-naphthyl) acetonitrile and its related substances

作　　者：张红霞[1] 徐利君[1] 黄荣清[1] 肖炳坤[1] 杨建云[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《现代仪器》2010年第1期19-21,24,共4页Modern Instruments

基　　金：国家科技重大专项"重大新药创制"综合性新药研究开发技术平台(No.2009ZX09301-002)资助;国家自然科学基金项目(No.20672143)

摘　　要：目的:采用反相高效液相色谱法测定(7-甲氧基-3,4-二氢-1-萘基)乙腈含量及其有关物质。方法:采用Diamonsil^(TM)(钻石)C_(18)(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(68:32),检测波长232nm,流速0.8mL/min。结果:此色谱条件下(7-甲氧基-3,4-二氢-1-萘基)乙腈与有关物质能达到有效分离,在40.48～161.92 μ g/mL范围内线性关系良好,r=0.9998;仪器精密度RSD为0.94%;方法重复性RSD为1.3%;最低检测限4.9ng。结论:该方法简便、准确、可行,可用于(7-甲氧基-3,4-二氢-1-萘基)乙腈的质量控制。Objective： A reversed phase high performance liquid chromatography method for the determination of content of （7 - methoxy-3,4 - dihydro -1 - naphthyl） acetonitrile and its related substances. Methods： The analysis was performed on Diamonsil C18 reversed - phase analytical column（250mm ×4.6mm,5μm）;the mobile phase con- sisted of a mixture of methanol -water（68 ： 32）; The ultraviolet detection wavelength was set at 232nm.The flow rate was 0.8 mL/min. Results： （7 - methoxy-3,4 - dihydro -1 - naphthyl） acetonitrile and its related substances could be seperated well. The linearity of the （7 - methoxy-3,4 - dihydro -1 - naphthyl） acetonitrile curve was well correlated （r=0.9998） within the range of 40.48-161.92μg/mL.The instrument precision RSD was 0.94%; The method preci- sion RSD was 1.3%; The detection limit was 4.9ng. Conclusions： The method appeared to be simple, accurate and reproducible; It can be used to control the quality of （7 - methoxy-3,4 - dihydro -1 - naphthyl） acetonitrile.

关 键 词：反相高效液相色谱法(7-甲氧基-3  4-二氢-1-萘基)乙腈含量测定有关物 质7 氧基四氢萘-1-酮 

分 类 号：O657.72[理学—分析化学]