樱草杜鹃黄酮类成分的提取工艺研究  

Optimal technology for extracting flavonoids from Rhodendron primulaeflorum Bur.et Franch.

作　　者：谢明德[1] 吴娟[1] 彭括 周先礼[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]西藏自治区藏药厂,西藏拉萨850000

出　　处：《中国医药导报》2010年第18期67-68,共2页China Medical Herald

基　　金：国家自然科学基金(项目编号:20872122)

摘　　要：目的:优选樱草杜鹃中总黄酮的最佳提取工艺。方法:以芦丁为评价指标,考察黄酮质量分数,通过正交试验对超声法提取黄酮的工艺进行优化。结果:超声提取法的最佳工艺条件为以樱草杜鹃叶为原料,75%乙醇为提取液,m(樱草杜鹃)∶v(提取液)=1∶10,超声时间为60min,提取3次;在该条件下,总黄酮的提取率为11.01%,RSD为0.43%(n=3)。结论:优选了樱草杜鹃总黄酮的最佳提取工艺,提高药材的利用率,为更好地利用该药材提供了理论依据。Objective：To optimize the best extraction technology of flavonoids in leaves of Rhodendron primulaeflorum Bur.et Franch.Methods：For the evaluation of rutin to study the mass fraction of flavonoids,ultrasonic extraction process was optimized flavonoids by orthogonal test.Results：The optimum ultrasonic extraction conditions as follows：the Rhodendron primulaeflorum Bur.et Franch.leaves was as raw material,75% ethanol for the extract,m（Rhodendron primulaeflorum Bur.et Franch.）：v（extract） was 1：10,ultrasonic was 60 min,extracted three times;in this condition,the extraction rate of flavonoids was 11.01% with a RSD of 0.43%（n=3）.Conclusion：Optimize the best extraction technology of flavonoids in Rhodendron primulaeflorum Bur.et Franch.leaves and improve the utilization of medicinal herbs in order to better use of the medicine provides a theoretical basis.

关 键 词：樱草杜鹃 分光光度法 正交试验 黄酮 提取工艺 

分 类 号：R943[医药卫生—药剂学]