昆明汉族人群肾素-血管紧张素系统基因多态性与糖尿病肾病的相关性研究  

Association of the gene polymorPhisms in the renin-angiotensin system with diabetic nephropathy

作　　者：陈晓蕾[1] 罗平[1] 张建伟[1] 高揆[1] 周加莉[1] 

机构地区：[1]昆明市第一人民医院内分泌科,650011

出　　处：《中国糖尿病杂志》2010年第6期434-437,共4页Chinese Journal of Diabetes

摘　　要：目的探讨昆明地区汉族人群血管紧张素原(AGT)基因及血管紧张素转换酶(ACE)基因多态性与糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测T2DM不伴DN患者74例(T2DM组)、DN患者120例(DN组)及60名健康体检者(对照组)的ACE I/D及AGT M235T基因型。结果 (1)DN组ACE-DD基因型频率和D等位基因频率(分别为53%、71%)均高于T2DM组(分别为22%、46.6%)及对照组(分别为29%、45.5%)(P均<0.01)。(2)AGT M235T基因型频率和等位基因频率三组差异无统计学意义(P>0.05)。(3)同时携带ACE-DD及AGT-TT基因型者发生DN的OR值为6.183。结论 (1)ACE-DD基因型和D等位基因可能是昆明地区汉族人群DN的易感因素。(2)未发现AGT M235T多态性单一因素与DN的发生有关联。(3)ACE-DD基因型与AGT-TT基因型可能在DN发生中有协同作用。Objective To investigate the relationships of gene polymorphisms of angiotensinogen （AGT） and angiotensin converting enzyme （ACE） with type 2 diabetic nephropathy（DN）. Methods The study included 74 T2DM patients without DN（T2DM）, 120 patients with DN（DN） and 60 healthy controls. The ACE I/D and AGT-M235T polymorphisms were detected by polymerase chain reaction （PCR） coupled with restriction fragment length polymorphism （PCR-RFLP）. Results ① The frequencies of ACE-DD genotype and ACE D allele were significantly higher in DN group than in T2DM and control groups （P〈0. 01, respectively）.②No significant differences were found in AGT-M235T polymorphism among three groups（P〉0. 05）. ③The DN OR associated with a combined genotype（DD＋ TT） was 6. 183. Conclusions ①ACE-DD genotype and D allele may be a risk factor for DN in Kunming Han nationality. ②There may be no association between AGT-M235T polymorphism and the initiation of DN. ③A synergistic relationship between ACE-DD and AGT M235T-TT genotype may have an effect on DN in T2DM.

关 键 词：血管紧张素原 血管紧张素转换酶 多态性 基因 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌]