在线富集-毛细管区带电泳法测定牛奶中三聚氰胺  

Determination of Melamine in Milk by Capillary Zone Electrophoresis After On-line Enrichment

作　　者：闵盛[1] 汪洋[1] 杨小琪[1] 

机构地区：[1]成都市食品药品检测中心,成都610045

出　　处：《理化检验（化学分册）》2010年第6期604-606,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了在线富集毛细管区带电泳法测定牛奶中三聚氰胺的含量。系统地考察了毛细管区带电泳工作条件,选定的试验条件为:①磷酸盐缓冲溶液浓度为20 mmol.L-1;②pH值为3.0;③运行电压为30 kV;④检测波长为208 nm。在优化的试验条件下,三聚氰胺在8 min内达到基线分离,其线性范围为0.83～106.70 mg.L-1,检出限(3S/N)为0.2 mg.L-1。在制备供试液后不同时间进样测定,三聚氰胺迁移时间的相对标准偏差为0.72%,峰面积的相对标准偏差为1.02%,结果表明样品在8 h内稳定。A method for the determination of melamine in milk by capillary zone electrophoresis（CZE） with on-line enrichment was proposed.Through systematic study on the conditions of separation by CZE,optimum conditions were found as follows： ① concn.Of PBS： 20 mmol·L-1;② pH values of PBS： 3.0;③ moving voltage： 30 kV;④ wavelength of detection： 208 nm.Linear relationship between values of detection signal and concentration of melamine was obtained in the range of 0.83-106.70 mg·L-1.Detection limit（3S/N） found was 0.2 mg·L-1.Under the optimum condition,melamine was completely separated from the baseline within 8 min.Values of RSD′s of migration times and peak area were 0.72% and 1.02%,and as proved by the results obtained,the sample was stable within 8 h.

关 键 词：毛细管区带电泳 在线富集 三聚氰胺 

分 类 号：O657.8[理学—分析化学]