肝细胞生长因子修饰兔骨髓间充质干细胞的实验研究  

Hepatocyte growth factor modifies mesenchymal stem cells in rabbits

作　　者：周密[1] 王岩[2] 张树明[1] 汪劲松[3] 傅捷[1] 张莉[2] 徐建强[1] 娄晓[3] 郭子宽[3] 

机构地区：[1]第二炮兵总医院骨科,北京100088 [2]解放军总医院骨科,北京100853 [3]军事医学科学院放射与免疫研究所,北京100850

出　　处：《军医进修学院学报》2010年第7期721-723,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家"863"计划项目(2007AA02Z454)~~

摘　　要：目的研究重组腺病毒肝细胞生长因子对兔骨髓间充质干细胞的转染率及转染后细胞的分化特点。方法获取骨髓间充质干细胞并传代培养、鉴定,以MOI=150的重组腺病毒肝细胞生长因子(Ad-HGF)对其进行修饰,以Ad-GFP检测病毒72h转染率,观察转染后细胞的成骨及成脂分化特点。结果 Ad-HGF对MSCs感染效率为99.78%,成骨及成脂诱导14d后可见典型分化。结论以Ad-HGF对MSCs进行感染效果良好,可成为缺血性骨坏死较为有效的治疗手段。Objective To study the effect of transplantation of adenovirus recombinant hepatocyte growth factor （Ad-HGF） on transfection rate of mesenchymal stem cells （MSC） and their differentiation features after transfection in rabbits. Methods Harvested MSC were isolated, identified, sub-cultured, and modified when MOI=150. Transfection rate was detected with Ad-GFP and characteristics of osteoblasts and lipoblasts were observed 72h after transfection. Results The infection rate of Ad-HGF for MSC was 99.78%. Typical differentiation of osteoblasts and lipoblasts was observed after 14 days. Conclusion MSC can be effectively infected with Ad-HGF, which can thus be used as an effective therapy for ischemic osteonecrosis.

关 键 词：肝细胞生长因子 骨髓 间质干细胞 兔 

分 类 号：R687.2[医药卫生—骨科学]