红细胞聚集性光反射法测量  

The measurement of erythrocyte aggregation via scattered light

作　　者：魏明[1] 席晓莉[2] 

机构地区：[1]第四军医大学生物医学工程系物理学教研室,陕西西安710033 [2]西安理工大学自动化与信息工程学院

出　　处：《第四军医大学学报》1999年第3期200-202,共3页Journal of the Fourth Military Medical University

摘　　要：红细胞聚集性在临床血液流变学研究中占重要地位，为了方便准确地测定反映该特性的参数，作者研制了一种自动测量聚集参数的仪器，系统由双圆筒切变仪、半导体激光器（波长λ=810um）和微机构成．测量原理在于分析血液受激光照射的反射光强，它反映了血液表层红细胞的流变特性．在受微机控制的从30S-1到500S-1共20种切变率条件下进行测量，可以测得六个聚集参数：初始聚集时间（Ta）、终末聚集时间（Tf）、结构参数（Is）、动态聚集指数（S10）、部分解聚阈值（γD）、完全解聚阈值（（γs）．此方法灵敏度高、重复性好、整个测量仅需3min．Erythrocyte aggregation remains a central issue in clinical hemorrheology. Thus an automatic instrument including Coutte shear s.ystem, a semiconductor (λ=810 nm) and single chip computer was designed for measuring aggregation parameters of red blood cells. The method is based on the measurement of scattered laser light which reflects the rheological behavior of erythrocyte near the blood surface. The measurements are performed with a small (0.3 mL ) blood sample at 20 computerized shearing rates from 30 s-1 to 500s-1. Six parameters can be obtained: Original aggregation time (Ta), Final aggregation time (Tf), Structure parameter (Is), Dynamic aggregation index (S10), Partly dissociation threshold (γD ) and thoroughly dissociation threshold (γD )'This method is sensitive, reproducible and can be preformed in less than 3 min.

关 键 词：红细胞聚集 单片机 测量 光反射法 血液流变学 

分 类 号：R312[医药卫生—基础医学] R331.11