用RT-PCR对mRNA进行定量分析  

Quantitative analysis of mRNA with RT-PCR

作　　者：戴建国[1] 肖华胜[2] 张萍[2] 陈镔复[2] 赵文明[1] 

机构地区：[1]西安交通大学生物科学与工程系分子生物学研究室,陕西西安710049 [2]第四军医大学神经科学研究所

出　　处：《第四军医大学学报》1999年第3期242-243,共2页Journal of the Fourth Military Medical University

摘　　要：编码蛋白质的mRNA通过反转录酶的作用，将mR－NA反转录成cDNA，再通过PCR扩增，DNA产物得到指数形式的增加．在一定循环数内，PCR产物的量与其模板cD－NA，也就是相应mRNA的浓度有关．PCR产物电泳后掺入溴化乙锭，可通过扫描荧光强度来确定PCR产物的量．通过优化实验条件，可计算出所检测的mRNA的相对含量．mRNA of encoding protein was reverse transcripted to be cDNA by polymerase chain reaction (PCR). With the enhancement of PCR,DNA production increased to exponential multiple. And the PCR production was related to the CDNA template or the concentration of mRNA during optimum cycles. After electrophoresis, stained ethidium bromide (EB) into PCR products,the quantity of PCR product was determined by scanning of fluorescent intensity. The relative concentration of mRNA can be calculated by optimizing the experimental condition.

关 键 词：RT-PCR 溴化乙锭 MRNA 聚合酶链反应 

分 类 号：Q522.2[生物学—生物化学]