枸杞总黄酮类化合物抗脂质过氧化研究  

The protective effects of total flavonoids from Lycium Barbarum L. on lipid peroxidation of liver mitochondria and red blood cell in rats

作　　者：黄元庆[1,2,3] 鲁建华[1,2,3] 沈泳[1,2,3] 卢景担 古丽 贺晨霞 

机构地区：[1]宁夏医学院营养与食品卫生教研室 [2]兰州医学院第二附属医院眼科 [3]北京医科大学天然与仿生药物国家重点实验室

出　　处：《卫生研究》1999年第2期115-116,共2页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：利用Ｆｅ２＋－Ｃｙｓ－Ｈｉｓ体系诱发鼠肝线粒体和红细胞产生脂质过氧化的方法，研究了枸杞总黄酮类化合物（ＴＦＬ）的抗脂质过氧化作用。结果表明，ＴＦＬ可阻断该体系诱发的鼠肝线粒体脂质过氧化，当ＴＦＬ终浓度在０．０２５～２．０ｍｇ／ｍｌ范围时，对丙二醛（ＭＤＡ）生成阻断率为３８．３８％～９９．９６％，呈剂量－效应关系，同时具有保持线粒体膜流动性的作用。扫描电镜观察到ＴＦＬ对脂质过氧化所致红细胞形态结构的破坏有保护作用。The protective effects of total flavonoids from Lycium Barbarum L.(TFL) on lipid peroxidation in mitochondria and red blood cells (RBC) induced by oxygen radicals produced by Fe 2+ cysteine system were investigated. The mitochondria lipid peroxidation (measured as malondialdehyde, MDA) was significantly inhibited by TFL with a dose response relation between the concentrations of 0 025 and 2 0 mg/ml, and the fluidity of mitochondria membrane was also protected effectively. It was observed by scan electron microscope, that the shape of RBC in the Fe 2+ system was damaged significantly. The shape of RBC was remained with the addition of TFL.

关 键 词：枸札总黄酮 线粒体 脂质过氧化 实验研究 

分 类 号：R285.5[医药卫生—中药学] R931.71[医药卫生—中医学]