支气管哮喘患者肺泡巨噬细胞释放内皮素-1的研究  

STUDY OF ENDOTHELIN 1 RELEASED BY ALVEOLAR MACROPHAGE IN PATIENTS OF BRONCHIAL ASTHMA

作　　者：李玉[1] 王淑贞[1] 吴大玮[1] 

机构地区：[1]山东医科大学附属医院内科

出　　处：《山东医科大学学报》1999年第1期47-48,共2页Acta Academiae Medicinae Shandong

基　　金：山东医科大学青年科研基金

摘　　要：为研究支气管哮喘患者肺泡巨噬细胞（Ａｍ）所释放的内皮素１（ＥＴ１）在哮喘发病中的作用，对发作期哮喘患者２０例，对照组８例，经纤支镜行支气管肺泡灌洗术获得Ａｍ，调整Ａｍ至１×１０６／ｍｌ后分３孔体外培养（１ｍｌ／孔）；生理盐水对照孔、氟美松孔（１μｇ／ｍｌ）、氨茶碱孔（１５μｇ／ｍｌ），培养６ｈ后取上清液，采用放免法测定ＥＴ１。显示哮喘组血浆ＥＴ１明显高于对照组，并于第１秒用力肺活量（ＦＥＶ１．０）呈显著负相关。哮喘组Ａｍ体外释放ＥＴ１与对照组无显著差异，与ＦＥＶ１．０无相关性。氟美松孔Ａｍ释放ＥＴ１明显低于生理盐水对照孔；氨茶碱孔与生理盐水孔或氟美松孔无差异。To study the significance of endothelin 1(ET 1) released by alveolar macrophages(AM) in patients of bronchial asthma plasma and AM were obtained 20 patients with asthma and 8 healthy control subjects and were cultivated in following 3 wells: Saline control well, theophyllin well (15μg/ml),dexamethasone well (1μg/ml) for 6 hours. Then a supernate was obtained to mesure the ET 1 by RIA. The plasma ET 1 of asthma patients was higher than that of the control and was significantly negatively correlated with the percentage of predicted FEV1.0. The ET 1 released by asthma AM was not correlated with the percentage of predicted FEV1.0. There was no difference in ET 1 released by AM between the asthma and control groups. The ET 1 released by AM of dexamethasones well was lower than that of saline control. There was no difference in ET 1 between the saline well and the theophylline well. It could be that ET 1 released by the AM played no significant role in the pathophysiology of asthma.

关 键 词：哮喘 内皮素1 肺泡 巨噬细胞 

分 类 号：R562.25[医药卫生—呼吸系统]