大黄酸对高糖和血管紧张素Ⅱ诱导的大鼠近端肾小管上皮细胞肥大的影响  

Effects of Rhein on the Hypertrophy of Renal Proximal Tubular Epithelial Cells Induced by High Glucose and AngiotensinⅡ in Rats

作　　者：余德芊[1] 高原[2] 刘晓红[1] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003 [2]遵义医学院珠海校区生理学教研室,广东珠海519041

出　　处：《中药材》2010年第4期570-574,共5页Journal of Chinese Medicinal Materials

基　　金：珠海市科技基金资助项目(PC20052031)

摘　　要：目的:探讨大黄酸(Rhein)对高糖和血管紧张素Ⅱ(angiotensin,AngⅡ)诱导的大鼠近端肾小管上皮细胞肥大的影响。方法:麻醉下,无菌分离大鼠单根近球小管并培养,以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞。在高糖(30 mmol/L)环境下用AngⅡ(10-7mol/L)刺激肾小管上皮细胞肥大。与此同时,加入不同浓度的大黄酸(30、15、5 mg/L),作用72 h后检测细胞体积、3H-亮氨酸掺入量以及蛋白质含量以观察细胞肥大的变化。结果:高糖(30 mmol/L)+AngⅡ(10-7mol/L)培养72 h导致细胞体积显著增大,3H-亮氨酸掺入量及细胞内蛋白质含量显著增加。加入大黄酸处理72 h后,大黄酸30 mg/L可显著降低高糖和AngⅡ所致的细胞体积增大、3H-亮氨酸掺入量及细胞内蛋白质含量的升高。大黄酸15 mg/L降低了3H-亮氨酸掺入量及细胞内蛋白质含量,而对细胞体积无显著抑制作用。大黄酸5 mg/L可降低细胞内蛋白质含量,而对细胞体积及3H-亮氨酸掺入量无显著抑制作用。结论:大黄酸能抑制高糖和AngⅡ诱导的肾小管上皮细胞肥大。Objective：To explore the effect of Rhein on the hypertrophy of renal proximal tubular epithelial cells induced by high glucose and angiotensin II in rats.Methods：Studies were performed on anesthetized SD rats.Renal proximal tubular were gained by microdissection and cultured in RPMI-1640 medium.The cell types were identified by immunocytochemistry.The renal proximal tubular epithelial cells were incubated with high glucose（30 mmol/L） and angiotensin II（10-7 mol/L） to induce the hypertrophy of cells.To observe the effect of Rhein on hypertrophy induced by high glucose and angiotensin II,renal proximal tubular epithelial cells were cultured with different concentrations of Rhein（30,15,5 mg/L） for 72 h,then cell size,3H-leucine incorporation,and cellular protein content were detected to observe the changes.Results： High glucose（30 mmol/L） and Ang II（10-7 mol/L） induced hypertrophy of renal proximal tubular epithelial cells result in,cell size,3H-leucine incorporation and cellular protein content increased significantly.On the contrary,Rhein inhibited the hypertrophy of renal proximal tubular epithelial cells induced by high glucose and AngiotensinⅡ.Rhein 30 mg/L significantly decreased cell size,3H-leucine incorporation and cellular protein content.Rhein 15 mg/L decreased 3H-leucine incorporation and cellular protein content.Rhein 5 mg/L decreased cellular protein content.Conclusions：Rhein can inhibit the hypertrophy of renal proximal tubular epithelial cells induced by high glucose and AngiotensinⅡ in rats.

关 键 词：大黄酸 高糖 血管紧张素Ⅱ 近端肾小管上皮细胞 细胞肥大 

分 类 号：R285.5[医药卫生—中药学]