银杏内酯B对大鼠脑缺血-再灌注损伤炎症反应的影响  

Effects of Ginkgolide B on Inflammation Induced by Cerebral Ischemia-Reperfusion in Rats

作　　者：刘永刚[1] 李芳君[1] 王婧[1] 王晓东[2] 

机构地区：[1]武警广东总队医院药剂科,广东广州510507 [2]广州市花城制药厂,广东广州510555

出　　处：《中药材》2010年第4期578-580,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:研究银杏内酯B对大鼠脑缺血-再灌注损伤后炎症的影响。方法:缺血前给予银杏内酯B 2.5、5、10 mg/kg,舌下静脉给药3 d,末次给药1 h后制备大鼠大脑中动脉阻断短暂局灶性缺血模型。脑缺血2 h再灌注24 h后,用TTC染色法、伊文思蓝法(EB)测定脑梗死范围及血脑屏障的损伤程度。分别用放射免疫、酶免疫法测定大脑缺血区肿瘤坏死因子α(TNF-α)、血清白介素6(IL-6),血清白介素1β(IL-1β)的含量和E选择素、细胞间黏附分子1(ICAM-1)表达。结果:银杏内酯B能减小脑缺血-再灌注后脑梗死百分比,减轻神经损伤症状,降低脑内TNF-α及血清IL-6,IL-1β含量及E选择素、ICAM-1的表达。结论:银杏内酯B可通过抑制脑缺血-再灌注过程中炎症介质的释放,降低脑缺血-再灌注所致的脑损伤。Objective：To investigate the protective effects of Ginkgolide B on inflammation induced by cerebral ischemia-reperfusion in rats.Methods：Rats were pretreated with Ginkgolide B at the dose of 2.5,5,10 mg/kg for 3 days and then subjected to cerebral ischemia/reperfusion induced by a middle cerebral artery occlusion（MCAO）.The infarct volume and the neurological deficit were determined by the method of TTC（2,3,5-triphenylterazolium chloride） staining and Longa′s score.The permeability of blood-brain barrier（BBB） was evaluated by measurement of the evans blue（EB） content in the brain with spectrophotometer.The content of interleukin-1β,interleukin-6（IL-6,IL-1β）in serum and tumor necrosis factor-α（TNF-α）,The content of E-selectin and ICAM 1 in brain were determined by radio-immunoassay and ELISA assay.Results：Ginkgolide B reduced infarct volume,ameliorated the neurological deficit and the permeability of BBB、the content of IL-6、IL-1β in serum and TNF-α、expressions of E-selectin and ICAM 1 in brain tissue also were significantly decreased.Conclusion： Ginkgolide B has protective effects on cerebral injury by inhibiting the inflammation induced by ischemia/reperfusion injury.

关 键 词：银杏内酯B 白介素1Β 白介素6 细胞间黏附分子1 脑缺血 

分 类 号：R285.5[医药卫生—中药学]