响应面分析法优化乳苣总黄酮提取工艺的研究  

Optimization of the Extraction Technology of Total Flavonoids from Mulgedium tataricum Using Response Surface Analysis

作　　者：高义霞[1] 景红艳[1] 姜祖君[1] 张继[1,2] 杨声[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001 [2]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《中药材》2010年第4期621-624,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:利用响应面法优化乳苣总黄酮的提取工艺条件。方法:以提取溶剂乙醇浓度、液固比、提取温度及提取时间为影响因子,应用Box-Behnken中心组合设计建立数学模型,以提取总黄酮得率作为响应值,进行响应面法分析(RSM)。结果:乳苣总黄酮提取的最佳工艺为:乙醇浓度63.78%,液固比40∶1,提取温度90℃,提取时间1.07 h,理论提取率14.04%,实际测得值为13.79%,两者较接近。结论:Box-Behnken设计结合响应面分析法可以很好地对乳苣总黄酮提取工艺进行优化。Objective： Response surface analysis methodology（RSM） was used to optimize the extraction technology of total flavonoids from Mulgedium tataricum.Methods： Ethanol concentration,solvent-solid ratio,extraction temperature and extraction time were selected as influencing factors during extraction.The experiment mathematical model was arranged according to Box-Behnken central composite experiment design.Results： The optimal extraction conditions were as following： ethanol concentration 63.78%,solvent-solid ratio 40∶1,extraction temperature 90 ℃,and extraction time 1.07 h,the predicted value of extraction rate of total flavonoids was 14.04% under the optimum conditions,and the experimental value was 13.79%.Conclusion： The combination of Box-Behnken design and response surface analysis can well optimize the extraction technology of total flavonoids from Mulgedium tataricum.

关 键 词：乳苣 总黄酮 响应面分析法 

分 类 号：R284.2[医药卫生—中药学]