高效液相色谱法测定三羟乙基芦丁的血药浓度  

Determination of Troxerutin Concentration in Plasma by HPLC

作　　者：陈镇生[1] 

机构地区：[1]浙江省药品检验所

出　　处：《药物分析杂志》1999年第2期111-114,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立三羟乙基芦丁的血药浓度测定方法。方法：将三羟乙基芦丁转变成四羟乙基芦丁，经高效液相色谱分离后，用荧光检测器测定。结果：血药浓度标准曲线在１２５～４０ｎｇ·ｍＬ－１间，ｒ＝０９９９４；最低检测限为１ｎｇ·ｍＬ－１；高、中、低３种浓度平均回收率为９１５２％～９５８３％。日内和日间平均ＲＳＤ小于４３％和５２％。结论：本法可为羟乙基芦丁的药动学研究提供一检测方法。Objective: To establish HPLC method for determining troxerutin in human plasma. Method: Trihydroxyethyl rutoside was converted to tetrahydroxyethyl rutoside by 2-chlorethanol.then separated and determined by HPLC/fluorescence detection. Results: The linear range was between 12540 ngmL-1 with r=09994,and the lowest limit of detection was 1 ngmL-1.The average recovery within the above range was 9152 %9583 %.The RSD values of intraday and interday were lower than 43 % and 52 %,respectively. Conclusion: The present study provides a reliable quantitative method for pharmacokinetic studies of troxerutin.

关 键 词：羟乙基芦丁 高效液相色谱法 血药浓度 

分 类 号：R972[医药卫生—药品] R969.1[医药卫生—药学]