HPLC法测定人血浆中氧化苦参碱含量  

Determination of Oxymatrine in Human Serum by HPLC

作　　者：孟根达莱[1,2] 王毅[1,2] 刘拴娣 石岩[1,2] 松布尔 

机构地区：[1]内蒙古医学院附属医院药局 [2]内蒙古包头市白音铁矿医院

出　　处：《药物分析杂志》1999年第2期114-117,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立测定氧化苦参碱血药浓度的ＨＰＬＣ法。方法：血浆标本经碱化以氯仿一正丁醇（９８∶２）提取，吹干后以甲醇一正己烷（４∶１，氨水调ｐＨ至８）为流动相，经过Ｓｐｈｅｒｉｓｏｒｂ－ＳｉＯ２（５μｍ）柱分离后在紫外２２０ｎｍ处检测。结果：氧化苦参碱血浆浓度曲线范围１２～３６ｍｇ·Ｌ－１，最低检出限为０１ｍｇ·Ｌ－１（Ｓ／Ｎ＝２）。样品在１２～３６ｍｇ·Ｌ－１浓度时的平均回收率为９８３１％～１０９０％，日内和日间精密度的ＲＳＤ均小于４％。结论：此方法为氧化苦参碱的血药浓度测定及药代力动学研究提供了一种简便可行的方法。Objective: To establish a HPLC method for determining oxymatrine in human plasma. Method: The plasma was extracted with chloroform-nbutyl alcohol(982) after basification.The oxymatrine was eluted with methanol-nhexane(41,adjusted to pH 8 with NH3H2O) on Spherisorb-SiO2 column and detected at 220 nm. Results: Calibration curve is linear from 1236 mgL-1.The limit of detection is 01 mgL-1(S/N=2).The range of mean recovery in samples of 1236 mgL-1 was 9831 %1090 % and the precision (RSD) of within-day and day-to-day was less than 4 % respectively. Conclusion: The present study provides a simple and reliable method for pharmacokinetic studies as well as for determining concentration of oxymatrine in human plasma.\=

关 键 词：氧化苦参碱 血药浓度 高效液相色谱 

分 类 号：R969.1[医药卫生—药理学]