用多孔微载体Cytopore高密度培养CHO工程细胞生产rHEPO  

The Production of rHEPO via High Density Cultivation of Recombinant CHO Cell Line by Using Porous Microcarrier Cytopore

作　　者：丛春水[1] 邓继先[1] 肖成祖[1] 程萱[1] 苏治国 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《生物技术通报》1999年第1期40-42,共3页Biotechnology Bulletin

摘　　要：在２．２Ｌｃｅｌｉｇｅｎ灌注培养系统中采用Ｃｙｔｏｐｏｒｅ多孔微载体培养产重组人促红细胞生成素（ｒＨＥＰＯ）的细胞Ｃ２。细胞密度达到１×１０７／ｍＬ，ｒＨＥＰＯ最高可达９．７ｍｇ／Ｌ。该载体具有表面积高，使用浓度低，便于扩大培养的特点。Using porous microcarrier Cytopore, a recombinant CHO cell line C2 producing have been successfully cultivated in 2.2 celligen perfusion culture system. The density of the cells could exceed 1×107 cells/ml. The yields of EPOreached 9.7mg/L. This porous microcarrier has the advantage of high surface, low using concentration, easy-to enlarge in large scale culture.

关 键 词：多孔微载体 CHO细胞 RHEPO 

分 类 号：TQ464[化学工程—制药化工]