重组分泌型人生长激素的纯化  

Purification of Recombinant Secretory Human Growth Hormone(rhGH)

作　　者：王宣军[1] 王冬倩[1] 刘欣荣[1] 王志武[1] 蒙冲[1] 郭凤云 李武平[1] 刘柱[1] 刘畅[1] 姚为民[1] 盛君[1] 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1999年第1期18-19,38,共3页Chinese Journal of Biologicals

摘　　要：rh GH工程菌 W310 0发酵培养后 ,经低渗裂解、阴离子交换柱层析、疏水层析、分子筛层析 ,得到纯化的人生长激素 ,纯度为 97%以上 ,比活大于 2 .5 IU /After secretory human growth hormone(rhGH) engineering bacterial strain W3100 was cultured in fermenter, cells were lysated by osmotic shock method, then rhGH was purified by ion exchange, hydrophobic and molecular size charomotagraphys. The purity and specific activity of the purified rhGH reached above 97% and 2.5 IU/mg respectively.

关 键 词：RHGH 纯化 人生长激素 

分 类 号：TQ464.7[化学工程—制药化工]