防龋卵黄抗体的研制  

Preparation of Yolk Antibody to Dental Caries

作　　者：姜崴[1] 赵小琳[1] 宋昌玲[1] 刘德宣[1] 曾繁启[1] 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1999年第1期23-25,共3页Chinese Journal of Biologicals

摘　　要：应用本室构建的龋齿疫苗工程菌株及 GTF抗原免疫母鸡 ,取鸡卵黄以水 -硫酸铵 -离子交换层析法提取纯化蛋黄抗体 (Ig Y ) ,以放免法测定血清及蛋黄中的抗体。结果用上述 2种抗原免疫的鸡 ,在血清及蛋黄中均能检出抗 GTF抗体。提纯的蛋黄抗体 ,经 SDS- PAGE电泳检测 ,相对分子质量约为 2 2 0 0 0 0 ,纯度达到 97.9% ,经 2 -巯基乙醇处理后 ,分为重链和轻链 ,相对分子质量分别约为 76 0 0 0和 2 80 0 0。经免疫印迹分析 ,具有良好的免疫学特异性。Yolk antibody(IgY) was purified from the eggs yolk laid by the hens immunized with the dental caries vaccine DCV strain(constructed by the authors) or glucosyltransferase(GTF) antigen(extracted from DCV strain) by water extraction, salting out with ammonium sulfate and DEAE Sepharose column chromatography. The antibodies to GTF were detected out by RIA method both in the serum and eggs of the hens. SDS PAGE showed that the molecular weight of the purified IgY was about 220 000, and the purity of it reached 97.9%. After being treated with 2 mercaptoethanol, the IgY was dissociated into a heavy(76 000) and a light(28 000) chains. Western blot showed the good immunological specificity of the IgY.

关 键 词：卵黄抗体 龋齿 葡糖基转移酶 

分 类 号：R988.2[医药卫生—药品] TQ929[医药卫生—药学]