大鼠染色体的非组蛋白、核糖核蛋白(RNP)与银染蛋白三者关系的研究  

Study on the Relationship of Nonhistone, RNP and Ag staining Protein of Rat Chromosome

作　　者：董洁[1] 卢静[1] 杨果杰[1] 王钜[1] 

机构地区：[1]首都医科大学医学实验动物部

出　　处：《实验动物科学与管理》1999年第1期27-30,共4页Laboratory Animal Science & Administration

摘　　要：本文应用ＴＥＭ技术、电镜细胞化学、胶体金标记和银染技术结合研究了大鼠（Ｒａｔｕｓｎｏｒｖｅｇｉｃｕｓｖａｒ．ａｌｂｕｌｕｓ）骨髓细胞染色体骨架。超薄切片结合ＲＮＰ优先染色法在染色体原位显示ＲＮＰ，同时又结合银染电镜技术及ＲＮａｓｅ－胶体金定位显示ＲＮＡ，结果显示金颗粒与银颗粒在染色体中分布的位置与ＲＮＰ所在部位恰好一致。说明ＲＮＰ中的蛋白质含有银染的酸性蛋白，即所观察到的染色体骨架非组蛋白、ＲＮＰ中的蛋白部分、银染蛋白三者是一致的。The chromosome scaffold of rat ( Rattus norvegicus var. albulus ) was studied by the combination of TEM, cytochemical EM, RNase gold complexes labeling and Ag staining technologies. RNP was shown in chromosomes in situ by the combination of ultra thin section and RNP preferred staining method. Meanwhile, RNA was shown by the combination of Ag staining TEM and RNase gold complexes labelling. The results showed that the localization of silver granules and gold particles in chromosome was the same as that of RNP. It indicated that Ag staining protein was nonhistone protein of chromosome scaffold and protein components of RNP, that is, nonhistone protein observed, protein components of RNP and Ag staining protein was the same.

关 键 词：染色体 染色体骨架 非组蛋白 RNP 银染蛋白 

分 类 号：Q959.837[生物学—动物学]