微束激光转基因技术研究进展  

The Developments about Laser Microbeam Transgenic Techniques

作　　者：付道林[1] 王兰岚[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《激光生物学报》1999年第1期70-75,共6页Acta Laser Biology Sinica

摘　　要：本文叙述了激光微速穿刺法导入外源ＤＮＡ的基本原理及其有关影响因素等。利用该转化方法，现已成功地获得了多种动植物细胞外源基因的转化。实验证明，激光微束穿刺转化技术简便有效、重复性好、靶体选择性强，对靶体无损伤等优点。随着转化技术自动化水平的提高。光镊技术的渗透和结合。In this riview ,the principle and several crucial factors of the osmsis-laser microbeam transformation system were summarozed .Exogenous genes have been successfully introduced into a number of animal and plant cells by this transformation system. It was proved that the osmosis-laser microbeam transformation technique was a potential and available method for transformation of foreign genes.Furthermore ,the method has many advantages ,for example ,it is simple ,effective ,precise ,selective and non-destructive to target cells.It is expected that laser microbeam in combination with optical tweezers and computer-control system will become a most useful and widely accepted way for transformation.

关 键 词：激光微束 转化 高渗缓冲液 

分 类 号：Q78[生物学—分子生物学]