乙型肝炎病毒HBsAg检测假阳性原因分析  

Analysis the cause of false positive results of HBsAg

作　　者：王娴默[1,2] 肖林[1,2] 范文[1,2] 张家均[1,2] 董虹[1,2] 雷选斌[1,2] 

机构地区：[1]长江大学附属第一医院 [2]湖北荆州第一人民医院,湖北荆州434000

出　　处：《国际检验医学杂志》2010年第5期423-424,共2页International Journal of Laboratory Medicine

摘　　要：目的我国人群乙型肝炎病毒(HBV)感染率高,HBV感染血清学标志物是临床实验室最主要的检测项目之一。本实验分析ELISA法检测HBV标志物s抗原(HBsAg)出现假阳性的原因并探讨应对措施。方法 ELISA法检测HBsAg阳性时,应用MEIA法复检。结果 156例样品用MEIA法复检HBsAgELISA法HBsAg阳性标本,结果4例阴性,ELISA法阴性误检率为2.56%。结论 ELISA法本实验室试剂存在HBsAg假阳性。在日常工作中,对ELISA法测定的HBV标志物5项指标HBsAg阳性的样品,严格多家试剂联合复检HBsAg是非常必要的。Objective Serological assay for hepatitis B virus（HBV）infection was one of important parameters in China because of high prevalence,We try to analyze the cause of false positive results of HBsAg during ELISA examination,and to find the possible solution.Methods Selected the positive specimen of HBsAg by ELISA,then examined them again by MELA.Results A-mong 156 sere positive of HBsAg reexamined by MEIA,4 specimen were negative,the false-positive rate was 2.56% for ELISA.Conclusion ELISA examination can lead to false-positive results of HBsAg,it is necessary to reexamine it with different reagents.

关 键 词：免疫测定 肝炎病毒 乙型 肝炎表面抗原 乙型 

分 类 号：R446.62[医药卫生—诊断学]