乙酰唑胺对大鼠精子发生和成熟的影响  

Effects of carbonic anhydrase inhibitor on rat spermatogenesis and sperm maturation

作　　者：舒如明[1] 王小强[1] 卢文红[2] 周善杰[2] 于和鸣[2] 谷翊群[2] 葛争艳[3] 

机构地区：[1]北京协和医学院国家人口计生委科学技术研究所,100081 [2]国家人口计生委科学技术研究所 [3]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中国计划生育学杂志》2010年第6期329-332,共4页Chinese Journal of Family Planning

基　　金：中央级公益性科研所基本科研业务费专项(2007计生所JZ01)

摘　　要：目的:研究碳酸酐酶抑制剂对大鼠精子发生和成熟的影响。方法:乙酰唑胺混悬液(40mg/kg)给大鼠灌胃,分别于给药当天、1d和3d处死动物,进行睾丸、附睾称重、苏木素-伊红(HE)染色和光镜下观察分析;附睾尾剪碎计数精子密度、精子活力,染色后计数精子畸形比率;测定附睾管腔液α-葡糖苷酶水平;采用罗丹明(Rh)123荧光探针标记附睾精子,通过流式细胞仪检测线粒体膜电位。结果:给予乙酰唑胺的大鼠精子活力明显降低,畸形率显著增加,附睾精子线粒体膜电位明显降低,附睾管腔液α-葡糖苷酶水平有所下降,但睾丸和附睾无明显病理学改变。结论:碳酸酐酶抑制剂可能通过影响附睾精子成熟,对雄性生殖功能进行调控。Objective：To study the effects of carbonic anhydrase inhibitor on spermatogenesis and sperm maturation.Methods：Rats were treated with acetazolamide（40mg/kg）on Day 0,Day 1 and Day 3 before they were sacrificed.The weights of the testis and epididymis were determined.The morphology observation of testis and epididymis were performed by hematoxylin-eosin staining and under light microscope.The sperm counts and sperm motility from the cauda of epididymis as well as the rate of sperm deformity were evaluated.The concentration of α-glycolsidase in epididymal luminal fluid was measured.The spermatozoa in epididymis were labeled with specific fluorescent probe of Rhodamine 123 to measure mitochondrial membrane potential（MMP）by flow cytometry.Results：After treatment with acetazolamide,the sperm motility and concentration of α-glycolsidase declined,and MMP of spermatozoa significantly decreased,but the rate of abnormal spermatozoa increased.However,the histological results of the testis and epididymis were remained unchangeable after acetazolamide treatment.Conclusion：The carbonic anhydrase inhibitor may regulate male reproductive function by interfering with the epididymal sperm maturation.

关 键 词：乙酰唑胺 睾丸 附睾 精子成熟 水通道蛋白-1 

分 类 号：R114[医药卫生—卫生毒理学]