pWX146-β-半乳糖苷酶融合蛋白表达载体的构建及对疟原虫基因的表达  

CONSTRUCTION OF A TRUNCATED β GALACTOSIDASE FUSION PROTEIN EXPRESSION VECTOR AND ITS APPLICATION IN HIGH EFFICIENT EXPRESSION OF HISTIDIN RICH PROTEIN Ⅱ (HRP Ⅱ)OF PLASMODIUM FALICIPARUM *

作　　者：王萍[1,2] 李明[1,2] 谢毅 殷明[1,2] 李英杰 

机构地区：[1]中国人民解放军第一军医大学热带病研究室 [2]上海复旦大学遗传所国家重点实验室基因合成室

出　　处：《寄生虫与医学昆虫学报》1999年第1期12-17,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：上海市青年科技启明星计划资助

摘　　要：本文根据抗原分子特性，用限制性内切酶ＨｐａⅠ和ＥｃｏＲⅠ及三对ＸｈｏⅠ－ＥｃｏＲⅠ接头，对β－半乳糖苷酶融合蛋白表达载体ｐＷＲ４５０进行了改建，截去其β－半乳糖苷酶Ｃ端约３００个氨基酸的编码序列，构建了一组含β－半乳糖苷酶Ｎ端１４６个氨基酸编码序列的ｐＷＸ１４６融合蛋白表达载体，以ｐＷＸ１４６－Ⅰ作为载体。We have constructed a pWX146 family based on pWR450,a β galactosidase fusion protein expression vector.The pWX146 family is consisted of pWX146,pWX146 1 and pWX146 2.Each of them includes a lac promoter of E.coli,a truncated sequence encoding 146 amino acids at the N terminus of β galactosidase and a different poly linker sequence.A 306bp gene fragment,encoding repetitive region of histidine rich protein Ⅱ(HRP Ⅱ) of Plasmodium,falcipatum was cloned into pWX146 1 and efficiently expressed fusion protein after induction with IPTG in E.coli.The truncation of the sequence encoding β galactosidase minimized influence on the stucture and expression of the expected target peptides,therefore the pWX146 family may also be useful for epitope analysis using sequential deletion strategy.

关 键 词：融合蛋白 表达载体 疟原虫 

分 类 号：R382.31[医药卫生—医学寄生虫学] Q78[医药卫生—基础医学]