液相培养法培养表达猪囊尾蚴融合蛋白的实验研究  

AN EXPERIMENTAL STUDY ON THE FUSION PROTEINS OF CYSTICERCUS CELLULOSE BY FLUID CULTURE AND EXPRESSION

作　　者：王敏[1] 王凯慧[1] 李雅杰[1] 徐之杰[1] 

机构地区：[1]哈尔滨医科大学寄生虫学教研室

出　　处：《寄生虫与医学昆虫学报》1999年第1期31-34,共4页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：应用已有的猪囊尾蚴２８ＫＤａ、１８ＫＤａ、１４ＫＤａ和３４ＫＤａ抗原基因，与λｇｔ１１重组后，以液相培养法转染Ｅ．ＣｏｌｉＹ１０９０，培养表达的融合蛋白以Ｄｏｔ－ＥＬＩＳＡ法检测囊虫病人血清，其中１８ＫＤａ片段经表达后效果最好，４种片段以等比联合应用检出率最高。与粗抗原进行的ＥＬＩＳＡ、ＩＨＡ相比，具有高度的特异性及较好的敏感性，并可解决抗原来源。We practised Cysticercus cellulose 28KDa 18KDa 14KDa and 34KDa antigen that had been obtained by the cDNA library and recombined them with λ gtll,then the recombinant phages infected E.Coli Y1090 by fluid culture to express fusion proteins.We tested with the cultured fusion proteins by Dot ELISA,the effect that tested with the fusion protein of 18 KDa phages showed best,The positive rate was the highest when testing with the four phages in equal proportion.Comparing with crude antigen by ELISA and IHA,there were highly specific and better sensitive to antibody.The study indicated that this method can solve the problem of source and purity of antigen.

关 键 词：猪囊尾蚴 液相培养 融合蛋白 DOT-ELISA 

分 类 号：R383.34[医药卫生—医学寄生虫学]