贵州四个山羊品种mtDNA多态性及起源分化  

MITOCHONDRIAL DNA POLYMORPHISM OF GUIZHOU GOAT BREEDS

作　　者：贾永红[1,2,3] 史宪伟[1,2,3] 简承松 朱文适[1,2,3] 张亚平 何正权[1,2,3] 廖正录[1,2,3] 余应淮 李通权[1,2,3] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室 [2]贵州大学南区动物科学系 [3]贵州省农业厅畜牧局

出　　处：《Zoological Research》1999年第2期88-92,共5页动物学研究（英文）

基　　金：云南省科委和贵州省科委资助项目

摘　　要：采用１５种限制性内切酶，研究贵州省４个山羊（ｃａｐｒａｓｐ．）品种共９３只个体的线粒体ＤＮＡ多态性。其中ＢａｍＨⅠ、ＨｉｎｄⅢ和ＳａｌⅠ３种酶的酶切类型存在多态。共检测到１８种限制性态型，归结为３种ｍｔＤＮＡ单倍型。单倍型Ⅰ和Ⅱ在贵州山羊４个品种中分布频率较高，分别为７７４２％和２１５０％，单倍型Ⅲ分布频率较低（１０８％）；品种间亲缘关系聚类分析表明白山羊和黑山羊亲缘关系最近，其次为黔北麻羊，而与小香羊的亲缘关系最远。推测贵州山羊ｍｔＤＮＡ单倍型Ⅰ和Ⅱ可能来源于两个母系祖先，其分化时间约在１９万年前。In this study,15 restriction enzymes were employed to investigate the mitochondrial DNA polymorphism among 93 individuals from 4 Guizhou goat breeds.Mitochondrial DNA polymorphism was detected by 3 enzymes ( Bam HⅠ, Hin dⅢ and Sal Ⅰ).18 restriction morphs,which could be sorted into 3 mtDNA haplotypes,were found.Haplotype Ⅰ and Ⅱ are distributed in every breed with relatively high frequency,i.e.77 42% and 21.50%.The frequency of haplotype Ⅲ is lower (1 08%).The relationship between white goat and black goat is the closest,and they are related to ma goat.Our results suggest that haplotype Ⅰ and Ⅱ of Guizhou goat's mtDNA might represent two maternal origin,which might separate about 190 thousand years ago.

关 键 词：山羊 MTDNA RFLP 起源分化 

分 类 号：Q959.842[生物学—动物学]