家蚕滞育的分子机理2.蛹期滞育激素基因的表达与滞育决定  

Molecular Mechanism of Diapaues in Bambyx mori2.Diapause Determination and Expression of Diapause Hormone Gene in Pupal Stage

作　　者：徐卫华[1] 

机构地区：[1]中国科学技术大学生物学系,安徽合肥230026

出　　处：《Acta Genetica Sinica》1999年第2期107-111,共5页

基　　金：中国科学院"九五"生物科学特别资助基金;中国科学院"九五"青年基金

摘　　要：滞育激素是由食道下神经节分泌的重要昆虫神经肽，诱导昆虫的滞育。选择具有ＲＮＡ聚合酶能够识别的启动子的质粒载体，将滞育激素ｃＤＮＡ克隆进去，在体外大量合成单一的滞育激素ｃＲＮＡ为参照，测定食道下神经节分泌滞育激素ｍＲＮＡ量来确定滞育激素的分泌量。结果证明食道下神经节分泌的滞育激素的数量决定昆虫的滞育。mbryonic diapause in Bombyx is induced by diapause hormone(DH),which isproduced in the suboesophageal ganglion from a precursor protein.We have clonedthe DH cDNA into pBluescript KS vector that containing T7 RNA promoter can beused to synthesize nonspecific RNA.DH cRNA was synthesized and used as internalcontrol.We quantitatively measured DH mRNA at SG of day 3 pupae by Northemhybridization analysis and suggested that the embryonic diapause of Bombyx in nextgeneration was determined by DH mRNA content in SG.

关 键 词：滞育激素 基因表达 滞育决定 家蚕 

分 类 号：S881.2[农业科学—特种经济动物饲养]