棉花合子期化学诱变获得的早熟品系及其RAPD分析  

Early Maturing Lines Obtained Through Chemical Mutagenesis onZygote in Cotton and Their RAPD Analysis

作　　者：沈法富[1] 于元杰[1] 尹承佾[1] 

机构地区：[1]山东农业大学农学系,山东泰安271018

出　　处：《Acta Genetica Sinica》1999年第2期174-178,共5页

摘　　要：报道了在棉花自花授粉后，利用花粉管通道将０．５ｍｍｏｌ／ＬＮａＮ３直接导入鲁锦６号的胚囊中，在变异后代中，采取系统选择的方法，选育出了一个早熟品系，它的熟期比鲁棉６号提前２５天，并且性状稳定。经过时鲁棉６号和早熟株系进行ＲＡＰＤ分析，结果表明，ＮａＮ３可以引起后代ＤＮＡ序列的广泛变异，并且棉花基因组可能存在着诱变剂作用的热点。his study reports that 0.5mmol/L NaN3 was introduced into Lumian No.6directly through pollen tube after selt-pollination of cotton An early maturing plantline was obtained from their varied offsprings using pedigree method. It matured 25dags earlier than that cf Lumian No.6 The line stabililed in character. Through RAPDanalysis (Random Amplified Polymorphic DNA) of the Lumian No.6 and earlymaturing line. the results indicated that the great variation of DNA sequence ofoffsprings could be caused ky treating zygoted with NaN and the hot site mutated bymutagenic agent may be existence in cotton genome.

关 键 词：棉花 合子 诱变育种  RAPD 

分 类 号：S562.035.2[农业科学—作物学]